µ-Slide VI - Flat

可平行測試 6 種實驗條件，每個 channel 僅需 3–9 x 10⁴ 細胞與 30 µl 試劑。
從細胞培養、染色到顯微鏡觀察，一氣呵成使用超便利！
微通道結構可避免因表面張力產生弧形液面的現象，細胞影像清晰不變形。
ibiTreat 底部經特殊處理，適合各類細胞貼附生長，免去 coating 麻煩。
適合貼覆性細胞的短時間影像觀察與免疫螢光染色 (immunofluorescence) 實驗。

歡迎與我們聯繫索取更多 µ-Slide VI - Flat 產品資訊與文獻。

產品特點

從細胞培養、染色到顯微鏡觀察“All-in-one”完成，一氣呵成高效便利

Easy Immunofluorescence Protocol | Also Suitable for use with Upright Microscopes

微通道結構可避免因表面張力產生弧形液面的現象，光線可筆直穿透，整個觀察視野亮度均勻，影像清晰不變形

Open well: The meniscus is disturbing the phase contrast effect. Only the center provides convenient contrast. ibidi Channel Slides: In a channel geometry the beam path is always aligned. Phase contrast microscopy is possible independent from the location.

底部厚度符合 #1.5 蓋玻片，影像清晰易觀測

Live cell phase contrast microscopy of Rat1 cells on a Collagen IV surface.

Triple immunofluorescence staining of MDCK cells. Mitochondria: MitoTracker™ (red); F-actin: Phalloidin (green); nuclei: DAPI (blue).

產品規格

µ-Slide VI 系列產品規格比較

	µ-Slide VI flat	µ-Slide VI 0.1	µ-Slide VI 0.4	µ-Slide VI 0.4 Bioinert	µ-Slide VI 0.5 Glass Bottom
Immunofluorescence assays	✓	✓	✓	✓	✓
Flow assays (cell culture under flow)	✕	✓	✓	✓	✓
3D cell culture	✕	✕	✕	✓	✕
Outer dimensions	W25.5 x L75.5 mm
Number of channels	6
Channel length	17 mm
Channel width	3.8 mm	1 mm	3.8 mm	3.8 mm	3.8 mm
Channel height	0.4 mm	0.1 mm	0.4 mm	0.4 mm	0.54 mm
Channel volume	30 µl	1.7 µl	30 µl	30 µl	40 µl
Growth area per channel	0.6 cm²	0.17 cm²	0.6 cm²	0.6 cm²	0.6 cm²
Coating area per channel	1.2 cm² (using 30 µl)	0.37 cm² (using 1.7 µl)	1.2 cm² (using 30 µl)	1.2 cm² (using 30 µl)	1.2 cm² (using 40 µl)
Volume per reservoir	None	60 µl	60 µl	60 µl	60 µl
Adapters	None	Female Luer	Female Luer	Female Luer	Female Luer
Bottom	ibidi Polymer Coverslip	ibidi Polymer Coverslip	ibidi Polymer Coverslip	ibidi Polymer Coverslip with Bioinert surface	Glass Coverslip No. 1.5H

底部材質規格比較

	#1.5 ibidi Polymer Coverslip (ibidi 特殊塑料)	#1.5H ibidi Glass Coverslip (#1.5H 高精度玻璃)
Bottom thickness	180 µm (+10/-5 µm)	170 µm (+/-5 µm)
Bottom material	Polymer	D 263 M Schott high precision glass
Gas permeability	Yes	No
Compatibility with protein coatings	Yes	Yes
Immersion oil compatibility	Yes	Yes
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Refractive index (nD 589 nm)	1.52	1.52
Brightfield Microscopy	++	++
Phase Contrast	++	++
Differential Interference Contrast (DIC)	++	++
Widefield Fluorescence	++	++
Confocal Fluorescence	++	++
Total Internal Reflection Fluorescence (TIRF)	+	++
Super-Resolution Microscopy	+	++

※更多材質特性比較資訊請見這裡。

訂購資訊

Product Name	Surface	Pack Size	Cat. No.
µ-Slide VI - Flat	ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized	15	IB-80626
µ-Slide VI - Flat	Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized	15	IB-80621