產品介紹細胞培養與分析細胞培養耗材細胞培養暨影像觀察兩用玻片/皿µ-Slide VI 0.4

µ-Slide VI 0.4

µ-Slide VI 0.4 - ibidi 台灣獨家代理伯森生技

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產品特點

從細胞培養、染色到顯微鏡觀察“All-in-one”完成,一氣呵成高效便利

The ibidi Channel Slides (e.g., µ-Slide VI 0.1 | µ-Slide VI 0.4 | µ-Slide VI 0.5 Glass Bottom | µ-Slide I Luer) are particularly suitable for immunofluorescence stainings. The geometry of the ibidi Channel Slides is ideal for the exact exchange of small medium amounts, which is necessary during immunocytochemistry stainings. In addition, the coverslip bottom of the channel μ-Slides eliminates the need for additional coverslips.

微通道結構可避免因表面張力產生弧形液面的現象,光線可筆直穿透,整個觀察視野亮度均勻,影像清晰不變形

Open well: The meniscus is disturbing the phase contrast effect. Only the center provides convenient contrast. ibidi Channel Slides: In a channel geometry the beam path is always aligned. Phase contrast microscopy is possible independent from the location.

底部厚度符合 #1.5 蓋玻片,影像清晰易觀測

Super-Resolution Microscopy (STED) of the Actin Cytoskeleton. The µ-Slide VI 0.4 is compatible with super-resolution microscopy methods, such as simulated emission depletion (STED) microscopy. Using LifeAct-TagGFP2 Protein, the actin cytoskeleton can be visualized in detail. In this experiment, fixed Rat1 fibroblasts were incubated with LifeAct-TagGFP2 protein in a µ-Slide VI 0.4, ibiTreat. Microscopy was performed on the STEDYCON super-resolution STED nanoscopy system (Abberior Instruments GmbH, Göttingen, Germany) with a Plan-Neofluar 100x/1.4 objective lens.

 

Fluorescence Microscopy for Mitochondria Visualization. The µ-Slide VI 0.4 is compatible with a variety of fluorescence microscopy methods. In this experiment, Madin-Darby Canine Kidney (MDCK) cells were cultured in a µ-Slide VI 0.4 and their mitochondria were visualized using MitoTracker. Image key: Mitochondria (MitoTracker, red), Actin cytoskeleton (Phalloidin, green), nuclei (DAPI, blue).

產品規格

µ-Slide VI 系列產品規格比較

  µ-Slide VI - flat
µ-Slide VI
flat
µ-Slide VI 0.1
µ-Slide VI
0.1
µ-Slide VI 0.4
µ-Slide VI
0.4
µ-Slide VI 0.4 Bioinert
µ-Slide VI
0.4 Bioinert
µ-Slide VI 0.5 Glass Bottom
µ-Slide VI
0.5 Glass Bottom
Immunofluorescence assays
Flow assays (cell culture under flow)
3D cell culture
Outer dimensions W25.5 x L75.5 mm
Number of channels 6
Channel length 17 mm
Channel width 3.8 mm 1 mm 3.8 mm 3.8 mm 3.8 mm
Channel height 0.4 mm 0.1 mm 0.4 mm 0.4 mm 0.54 mm
Channel volume 30 µl 1.7 µl 30 µl 30 µl 40 µl
Growth area per channel 0.6 cm² 0.17 cm² 0.6 cm² 0.6 cm² 0.6 cm²
Coating area per channel 1.2 cm²
(using 30 µl)
0.37 cm²
(using 1.7 µl)
1.2 cm²
(using 30 µl)
1.2 cm²
(using 30 µl)
1.2 cm²
(using 40 µl)
Volume per reservoir None 60 µl 60 µl 60 µl 60 µl
Adapters None Female Luer Female Luer Female Luer Female Luer
Bottom ibidi Polymer
Coverslip
ibidi Polymer
Coverslip
ibidi Polymer
Coverslip
ibidi Polymer
Coverslip with
Bioinert surface
Glass Coverslip
No. 1.5H

底部材質規格比較

  #1.5 ibidi Polymer Coverslip
(ibidi 特殊塑料)
#1.5H ibidi Glass Coverslip
(#1.5H 高精度玻璃)
Bottom thickness 180 µm (+10/-5 µm) 170 µm (+/-5 µm)
Bottom material Polymer D 263 M Schott high precision glass
Gas permeability Yes No
Compatibility with protein coatings Yes Yes
Immersion oil compatibility Yes Yes
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Refractive index (nD 589 nm) 1.52 1.52
Brightfield Microscopy ++ ++
Phase Contrast ++ ++
Differential Interference Contrast (DIC) ++ ++
Widefield Fluorescence ++ ++
Confocal Fluorescence ++ ++
Total Internal Reflection Fluorescence (TIRF) + ++
Super-Resolution Microscopy + ++

※更多材質特性比較資訊請見這裡

訂購資訊

Product Name Surface Pack Size Cat. No.
µ-Slide VI 0.4 ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 15 IB-80606
90 IB-80606-90
Collagen I: #1.5 polymer coverslip, sterilized 15 IB-80609
Collagen IV: #1.5 polymer coverslip, sterilized 15 IB-80602
Poly-L-Lysine: #1.5 polymer coverslip, sterilized 15 IB-80604
Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized 15 IB-80601
產品文件與相關資源