µ-Slide VI 0.4 Bioinert

Bioinert 底部經特殊材質「Polyol hydrogel」處理，比一般 ULA (ultra low sttachment) 培養盤更加難以令細胞附著，特別適合球狀體細胞 (spheroids)、類器官 (organoids) 的長時間培養與觀測。
搭配 ibidi Pump 使用即可進行流體環境 3D 細胞培養實驗 (3D cell culture under flow)。
可平行測試 6 種實驗條件，每個 channel 僅需 0.9–2.1 x 10⁴ 細胞與 30 µl 試劑，有效節省細胞與試劑用量。
由低自發螢光材質製成，且底部厚度符合 #1.5 蓋玻片標準，可直接結合高倍率光學或螢光顯微鏡進行細胞影像觀察。

歡迎與我們聯繫索取更多 µ-Slide VI 0.4 Bioinert 產品資訊與文獻。

產品特點

Bioinert 系列產品底部完全不具附著性，特別適合 Spheroids, Organoids, Embryoid bodies 的 3D 細胞培養實驗

Left: The Bioinert surface is ideally suited for the short- and long-term 3D culture of spheroids and organoids. Since both specific and non-specific cell immobilization are inhibited, cells are forced into a suspended state, enabling formation of small and large spheroids. To assess the viability, the cells were stained using fluorescein diacetate (FDA), which marks viable cells, and propidium iodide (PI), which stains dead cells. Right: Frontal view and sideview of an FDA-PI-stained MCF-7 cell spheroid on the Bioinert surface. Confocal laser scanning images are projected from an overlay of the serial confocal sections of the FDA signal. Microscope: Zeiss LSM 710, Plan Apochromat objective lens 10x/0.45. Scale bar is 100 µm.

ibidi Channel Slides 微流體玻片讓流體環境細胞培養實驗變得更簡單，不需繁複的組裝步驟，隨取隨用且無培養液滲漏風險

操作流程：(1) 打開玻片包裝，將細胞種入；(2) 待細胞貼附長滿後，於玻片兩端接上幫浦；(3) 於顯微鏡下觀察即時或是終端細胞影像。

產品規格

µ-Slide VI 系列產品規格比較

	µ-Slide VI flat	µ-Slide VI 0.1	µ-Slide VI 0.4	µ-Slide VI 0.4 Bioinert	µ-Slide VI 0.5 Glass Bottom
Immunofluorescence assays	✓	✓	✓	✓	✓
Flow assays (cell culture under flow)	✕	✓	✓	✓	✓
3D cell culture	✕	✕	✕	✓	✕
Outer dimensions	W25.5 x L75.5 mm
Number of channels	6
Channel length	17 mm
Channel width	3.8 mm	1 mm	3.8 mm	3.8 mm	3.8 mm
Channel height	0.4 mm	0.1 mm	0.4 mm	0.4 mm	0.54 mm
Channel volume	30 µl	1.7 µl	30 µl	30 µl	40 µl
Growth area per channel	0.6 cm²	0.17 cm²	0.6 cm²	0.6 cm²	0.6 cm²
Coating area per channel	1.2 cm² (using 30 µl)	0.37 cm² (using 1.7 µl)	1.2 cm² (using 30 µl)	1.2 cm² (using 30 µl)	1.2 cm² (using 40 µl)
Volume per reservoir	None	60 µl	60 µl	60 µl	60 µl
Adapters	None	Female Luer	Female Luer	Female Luer	Female Luer
Bottom	ibidi Polymer Coverslip	ibidi Polymer Coverslip	ibidi Polymer Coverslip	ibidi Polymer Coverslip with Bioinert surface	Glass Coverslip No. 1.5H

底部材質規格比較

	#1.5 ibidi Polymer Coverslip (ibidi 特殊塑料)	#1.5H ibidi Glass Coverslip (#1.5H 高精度玻璃)
Bottom thickness	180 µm (+10/-5 µm)	170 µm (+/-5 µm)
Bottom material	Polymer	D 263 M Schott high precision glass
Gas permeability	Yes	No
Compatibility with protein coatings	Yes	Yes
Immersion oil compatibility	Yes	Yes
Autofluorescence	Low	Low
Transmission	Very high (even ultraviolet)	High (ultraviolet restrictions)
Refractive index (nD 589 nm)	1.52	1.52
Brightfield Microscopy	++	++
Phase Contrast	++	++
Differential Interference Contrast (DIC)	++	++
Widefield Fluorescence	++	++
Confocal Fluorescence	++	++
Total Internal Reflection Fluorescence (TIRF)	+	++
Super-Resolution Microscopy	+	++

※更多材質特性比較資訊請見這裡。

訂購資訊

Product Name	Surface	Pack Size	Cat. No.
µ-Slide VI 0.4 Bioinert	Bioinert: #1.5 polymer coverslip, surface passivation with Bioinert, sterilized	15	IB-80600