µ-Slides With Multi-Cell µ-Pattern

可使球狀體細胞 (spheroids)、類器官 (organoids) 間隔排列生長於玻片上，進行 3D 細胞培養與影像觀察。
具有 µ-Slide VI 0.4 與 µ-Slide 8 Well high 兩種產品款式可選。
µ-Slide VI 0.4 搭配 ibidi Pump 使用，可進行流體環境 3D 細胞培養 (3D cell culture under flow)。
由低自發螢光材質製成，且底部厚度符合 #1.5 蓋玻片標準，可直接結合高倍率光學或螢光顯微鏡進行細胞影像觀察。

本產品亦可提供客製化服務，歡迎與我們聯繫索取詳細資訊。

技術原理

ibidi µ-Patterning 技術原理是先將一般 µ-Slide VI 0.4 或 µ-Slide 8 Well high 產品底部以特殊材質「Polyol hydrogel」處理，使其形成完成不具附著性的 Bioinert 表面。接著以共價鍵結的方式將源自於纖連蛋白 (Fibronectin) 結合區域的 RGD 三胜肽 (Arg-Gly-Asp, RGD tripeptide) 固定在 Bioinert 表面，使其形成供細胞附著生長的區域；根據 RGD 鍵結區域的大小與排列，可形成各種不同圖案設計。以上圖為例，紅色 ibidi 字樣為表面鍵結有 RGD peptides 的 µ-Pattern 區域，黑色則為不具有附著性的 Bioinert 區域，可以觀察到細胞僅會在 µ-Pattern 區域附著生長。

產品特點

µ-Pattern 特寫

100 µm 直徑大小的圓形 RGD 鍵結區域以六角形網格排列於 Bionert 表面 (彼此間距 500 μm)。

底部厚度符合 #1.5 蓋玻片，影像清晰易觀測

Spheroid formation of NIH-3T3 cells (murine embryo fibroblasts), 14 days after seeding single cells in the µ-Slide VI 0.4 With Multi-Cell µ-Pattern. Phase contrast microscopy, 4x objective lens.

Spheroid formation of NIH-3T3 cells (murine embryo fibroblasts) 14 days after seeding single cells in the µ-Slide 8 Well With Multi-Cell µ-Pattern. Brightfield microscopy, 10x objective lens.

CAR-T Cell Killing Assays. RCC-26 cancer cells immobilized on multi cell pads. Effector T cells applied in a collagen I matrix (Collagen Type I, Rat Tail) induce apoptotic body formation of cancer cells.

Live cell imaging of RCC-26 (human renal carcinoma) cells for 24 hours in the µ-Slide VI 0.4 With Multi-Cell µ-Pattern using the ibidi Stage Top Incubator. Phase contrast microscopy, 20x objective lens.

RCC-26 (human renal carcinoma) cells 24 hours after seeding in the µ-Slide VI 0.4 With Multi-Cell µ-Pattern, low density seeding for 2D monolayer. Phase contrast microscopy, 60x objective lens.

Immunofluorescence staining of RCC-26 (human renal carcinoma) cells 24 hours after seeding in the µ-Slide VI 0.4 With Single-Cell µ-Pattern. Green: F-actin (phalloidin), red: α-Tubulin, blue: nuclei (DAPI). Widefield fluorescence microscopy, 60x objective lens.

Immunofluorescence staining of RCC-26 (human renal carcinoma) cells 24 hours after seeding in the µ-Slide VI 0.4 With Multi-Cell µ-Pattern. Green: F-actin (phalloidin), red: α-Tubulin, blue: nuclei (DAPI). Widefield fluorescence microscopy, 10x objective lens.

產品規格

µ-Slide geometry	See product page µ-Slide VI 0.4 or µ-Slide 8 Well high
Binding motif	RGD
Pattern shape	Circle
Diameter	100 µm
Pitch	500 μm
Pattern layout	Hexagonal
Number of patterns in µ-Slide VI 0.4	ca. 300 per channel
Number of patterns in µ-Slide 8 Well high	ca. 450 per well
Surface passivation	Bioinert

訂購資訊

Product Name	µ-Pattern	Surface	Pack Size	Cat. No.
μ-Slide VI 0.4 μ-Pattern RGD, cir100, pit500, hex	RGD, 100 µm circles, 500 µm pitch, hexagonal	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83602-S
μ-Slide VI 0.4 μ-Pattern RGD, cir100, pit500, hex	RGD, 100 µm circles, 500 µm pitch, hexagonal		10	IB-83602
μ-Slide 8 Well high μ-Pattern RGD, cir100, pit500, hex	RGD, 100 µm circles, 500 µm pitch, hexagonal	#1.5 polymer coverslip, micropatterned surface, surface passivation with Bioinert, sterilized	2	IB-83802-S
μ-Slide 8 Well high μ-Pattern RGD, cir100, pit500, hex	RGD, 100 µm circles, 500 µm pitch, hexagonal		10	IB-83802

哪些細胞適用 µ-Pattern RGD 產品？

基本上只要能在 Fibronectin 處理表面生長的細胞，都能適用於 µ-Pattern RGD 產品，目前測試過的細胞包含：A549 (human lung carcinoma), ARPE-19 (human retinal pigmented epithelium), BEAS-2B (human lung epithelium), CHO (Chinese hamster ovary), HEK-293 (human embryonic kidney), HeLa (human cervical carcinoma), HT-1080 (human fibrosarcoma), HuH-7 (human liver carcinoma), L929 (murine fibroblasts), MCF-10A (human breast epithelium), MDA-MB-231 (human breast carcinoma), MDA-MB-436 (human breast carcinoma), MDCK.2 (canine kidney cells), NCI-H441 (human lung adenocarcinoma), NIH-3T3 (murine fibroblasts), RCC-26 (human renal carcinoma)。建議可先購買 2 片小包裝產品，測試您細胞在 µ-Pattern RGD 產品的生長情形。