產品介紹產品介紹細胞培養與分析細胞培養耗材細胞培養暨影像觀察兩用玻片/皿µ-Slide Spheroid Perfusion

µ-Slide Spheroid Perfusion

µ-Slide Spheroid Perfusion - ibidi 台灣獨家代理伯森生技

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產品特點

µ-Slide Spheroid Perfusion 可搭配 ibidi Pump 使用,於流體環境進行 3D 細胞培養實驗

Optimal Nutrition Through Specialized Geometry. The µ-Slide Spheroid Perfusion is a specialized flow chamber for culturing free-floating 3D aggregates. It consists of 3 x 7 flat-bottomed wells, which are connected through a channel above. Each well forms its own niche, where the specimen is cultured. When applying perfusion through the channel, fresh medium continuously diffuses to the specimen. This ensures optimal nutrition and oxygen diffusion throughout the experiment without that the specimen is exposed to significant shear forces. The setup guarantees maximum of viability, but with minimum of shear stress for spheroids, organoids, or tissue.

µ-Slide Spheroid Perfusion 應用彈性多元

µ-Slide Spheroid Perfusion is available with three different surfaces (Bioinert, ibiTreat, Uncoated), and is suitable for a variety of applications.

µ-Slide Spheroid Perfusion 上蓋與底部厚度皆符合 #1.5 蓋玻片,影像清晰易觀測

Highly Improved Spheroid Growth Rates When Cultured Under Perfusion. L929 fibroblasts show spheroid formation in the µ-Slide Spheroid Perfusion, Bioinert, days 1–14, seeding concentration 5 x 10⁵ single cells/ml. Left: no perfusion, medium exchange every second day. Right: perfusion with the ibidi Pump System, 0.75 ml/min. Phase contrast microscopy, 10x objective lens, well diameter 800 µm.

 

(A) Spheroid Formation of MCF-7 Breast Cancer Cells. Single cell seeding of MCF-7 breast cancer cells with spheroid formation in the µ-Slide Spheroid Perfusion, Bioinert, 1 day after seeding. Seeding concentration 5 x 10⁵ cells/ml. Phase contrast microscopy, 10x objective lens. (B) Live/Dead Staining of a Long-Term Cultured Spheroid. Live/dead FDA/PI staining of an L929 spheroid in the µ-Slide Spheroid Perfusion, Bioinert, after 14 days in culture with perfusion using the ibidi Pump System, 0.75 ml/min. Green: living cells (fluorescein diacetate, FDA); red: dead cells (propidium iodide, PI). Widefield fluorescence microscopy, 10x objective lens. (C) Fluorescence Microscopy of Adherent Cells. Fluorescence imaging of L929 fibroblasts in the µ-Slide Spheroid Perfusion, ibiTreat, fixated 2 hours after seeding. Green: F-actin (phalloidin); blue: nuclei (DAPI). Widefield fluorescence microscopy, 10x objective lens. (D) Organoid Co-Culture of PDAC Cells and Fibroblasts*. Organoid co-culture of the human pancreatic cancer (PDAC) cell line PA-TU-8988T (green, stained with CellTracker™ Green) and the murine fibroblast cell line mPSC4 (red, stained with CellTracker™ Orange CMTMR) in the µ-Slide Spheroid Perfusion. The µ-Slide was covered with a 25 µm FEP foil for matching the refractive index closer to water during upright light sheet microscopy. *The image was acquired by S. Volkery at MPI Muenster with the M Squared Aurora Airy beam upright light sheet setup. The sample was provided by K. Roth, University Marburg, Germany.

產品規格

Outer dimensions W25.5 x L75.5 mm
Adapters Female Luer
Number of channels 3
Number of wells 3 x 7
Volume per well 3.5 μl
Well height (bottom niche) 0.4 mm
Well height (total) 1.3 mm
Well diameter (bottom) 0.8 mm
Channel volume (total) 45 μl
Channel height 0.2 mm
Channel width 1.0 mm
Growth area per well 0.5 mm²
Coating area using 3.5 µl 9.7 mm²
Volume per reservoir 60 μl
Top cover ibidi Polymer Coverslip
Bottom ibidi Polymer Coverslip

訂購資訊

Product Name Surface Pack Size Cat. No.
µ-Slide Spheroid Perfusion Bioinert: #1.5 polymer coverslip, surface passivation with Bioinert, sterilized 15 IB-80350
ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 15 IB-80356
Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized 15 IB-80351

流體環境 3D 細胞培養產品比較表

  µ-Slide Spheroid Perfusion
µ-Slide Spheroid Perfusion
µ-Slide III 3D Perfusion
µ-Slide III 3D Perfusion
µ-Slide I Luer 3D
µ-Slide I Luer 3D
Perfusion of samples
Defined shear stress on cell monolayers
(on gel matrix)
Gel matrices for 3D
Spheroids/organoids free floating in well inside gel matrix only inside gel matrix only
Suspension cells free floating in well inside gel matrix only inside gel matrix only
Adherent cells on coverslip inside or on gel matrix inside or on gel matrix
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