µ-Slide III 3D Perfusion
- 三條獨立微通道，每條通道含 2 個培養槽，可供注入 gel matrix (例如 Matrigel®, collagen) 形成一平整細胞基質膠層，搭配 ibidi Pump 使用即可進行流體環境 3D 細胞培養實驗 (3D cell culture under flow)。
- 上蓋與底部厚度皆符合 #1.5 蓋玻片標準，可直接結合高倍率光學或螢光顯微鏡進行細胞影像觀察。
- 產品應用包含：流體環境 3D 細胞培養、球狀體細胞 (spheroids)／類器官 (organoids) 長時間培養與觀測。
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產品特點
µ-Slide III 3D Perfusion 可搭配 ibidi Pump 使用，於流體環境進行 3D 細胞培養實驗
The μ-Slide III 3D Perfusion is an array of 6 wells where cells can be cultivated and, subsequently, investigated with microscopical methods. Two of the 6 wells respectively are connected by a channel. The channels can be connected to ibidi Pump System for perfusing the wells in order to perform long term cell culture assays with 3D structures.
µ-Slide III 3D Perfusion 應用彈性多元
Application Examples Using the µ-Slide 3D Perfusion: (i) 3D Matrix Culture: Single cells can be cultured and imaged in a 3D matrix, e.g., a collagen gel; (ii) Soft Matrix Culture: Adherent cells can be cultured and imaged on a soft matrix; (iii) Spheroid Culture: Spheroids can be cultured and imaged, e.g., in a gel sandwich; (iv) Tissue Sample Culture: Tissue samples can be cultured and imaged, e.g., in a gel sandwich; (v) Culture Without Matrix: Adherent cells can be seeded directly on the substrate without any matrix.
µ-Slide III 3D Perfusion 上蓋與底部厚度皆符合 #1.5 蓋玻片，影像清晰易觀測
This is an Islet of Langerhans, which contains the hormone-producing cells of the pancreas, cultured in the µ-Slide III 3D Perfusion. Cells were stained for insulin (cyan) to detect the beta cells of the islet, and for Somatostatin receptor 3 (SSTR3) to visualize the cilia of the islet (magenta). Data by Alexander Ahl, Department of Molecular Medicine and Surgery I, Karolinska Institutet, Stockholm, Sweden.
產品規格
| Outer dimensions | W25.5 x L75.5 mm |
| Adapters | Female Luer |
| Number of wells | 6 |
| Volume of wells | 30 µl |
| Ø Well | 5.5 mm |
| Well height (without channel) | 1.2 mm |
| Well height (with channel) | 1.7 mm |
| Growth area per well | 25 cm² |
| Number of channels | 3 |
| Total channel volume | 130 µl |
| Channel width | 1.0 mm |
| Volume per reservoir | 60 μl |
| Coating area using 30 µl | 0.29 cm² per well |
| Coating area using 130 µl | 2.4 cm² per channel |
| Top cover | No. 1.5 coverslip |
| Bottom | ibidi Polymer Coverslip |
訂購資訊
| Product Name | Surface | Pack Size | Cat. No. |
|---|---|---|---|
| µ-Slide III 3D Perfusion | ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized | 15 | IB-80376 |
| Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized | 15 | IB-80371 |
流體環境 3D 細胞培養產品比較表
 µ-Slide Spheroid Perfusion |
 µ-Slide III 3D Perfusion |
 µ-Slide I Luer 3D |
|
|---|---|---|---|
| Perfusion of samples | ✓ | ✓ | ✓ |
| Defined shear stress on cell monolayers | ✕ | ✕ | ✓ (on gel matrix) |
| Gel matrices for 3D | ✕ | ✓ | ✓ |
| Spheroids/organoids | free floating in well | inside gel matrix only | inside gel matrix only |
| Suspension cells | free floating in well | inside gel matrix only | inside gel matrix only |
| Adherent cells | on coverslip | inside or on gel matrix | inside or on gel matrix |
