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找出您實驗的最佳轉染試劑

友善列印TransIT®-mRNA

  • 低細胞毒性!可減輕試劑本身對細胞生長的影響性,降低實驗變異因素。
  • 良好的轉染效率!可有效地將 mRNA / viral RNA 樣品轉染至多種細胞類型,例如 A549, CHO-K1, COS-7, HEK 293, HeLa, HepG2, NIH 3T3, Vero 等…。
  • 適用於血清培養環境!可有效地在含血清的細胞培養環境中作用,轉染前不必更換培養基,使用更便利。
  • 應用範圍廣泛!可應用在單個或多個不同長度的 mRNA / viral RNA 轉染實驗,應用領域包含蛋白質表現、病毒顆粒生產等…。

 

產品效能:

  
The TransIT®-mRNA Transfection Kit Transfects GFP mRNA into DC 2.4 Dendritic Cells
. Using TransIT®-mRNA Transfection Kit, DC 2.4 cells were transfected with (A) 0.5 µg, (B) 1 µg and (C) 2.5 µg of capped and polyadenylated mRNA encoding GFP with 1 µl TransIT®-mRNA Reagent and 1 µl Boost. Cells were seeded overnight at 100,000 cells/well in 24-well plates.  Images were taken 10 hours post-transfection.
Data courtesy of Kyle Phua (Principal Investigator: Kam W. Leong), Duke University
        
Multiple Dendritic Cell Types Express GFP from mRNA Transfected by TransIT®-mRNA Transfection Kit
. Murine primary bone marrow derived dendritic cells (BMDC) and murine dendritic cells types (JAWSII and DC 2.4) were transfected with 1 µg of capped and polyadenlyated mRNA encoding GFP using a TransIT®-mRNA Reagent: Boost: mRNA ratio of 1:1:1 (µl:µl:µg). Primary BMDCs, JAWSII and DC 2.4 were seeded (80,000 cell/well) overnight in 24-well plates. Cells were assayed via flow cytometry 8 hours post transfection. Error bars represent the standard deviation of at least 3 separate experiments.
Data courtesy of Kyle Phua (Principal Investigator: Kam W. Leong), Duke University

        
High Efficiency and Low Toxicity Transfection Following 14 Consecutive Transfections with TransIT®-mRNA Transfection Kit.
Repeated daily transfections were performed in the same population of BJ fibroblasts using TransIT-mRNA Transfection Kit, Lipofectamine® RNAiMAX (Life Technologies) and Stemfect™ RNA Transfection Kit (Stemgent) - with a capped and polyadenylated EGFP mRNA incorporating pseudouridine and 5mC modified bases (Trilink Biotechnologies, Inc.). Multiple reagent-to-RNA ratios were tested and the optimal ratio is represented. Transfections were performed in 12-well plates using the indicated reagent-to-RNA ratios to deliver 1 µg of RNA. GFP and phase contrast images were taken in the same field of view everyday after transfection. Transfection efficiency was measured by flow cytometry on a Guava EasyCyte 5HT following 14 consecutive daily transfections (blue bars). Cell viability was determined using cell counts measured during flow cytometry (black line grey bars). Error bars represent the standard deviation of triplicate wells.

 

訂購資訊

Product Pack Size Number of Transfections in 12-well Plate Cat. No.
TransIT®-mRNA Transfection Kit 0.4 ml 200 MR-MIR2225
1.0 ml 500 MR-MIR2250
5 x 1.0 ml 2,500 MR-MIR2255
10 x 1.0 ml 5,000 MR-MIR2256

 

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