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友善列印TransIT® Lentivirus System

TransIT® Lentivirus System 含有 TransIT®-Lenti Transfection ReagentLentivirus Packaging Mix Powered by MISSION®,在優化的轉染條件下,具感染力的慢病毒 (lentivirus) 力價可高達 108。產品特點包含:

  • 高生產效率:病毒力價優於 Lipofectamine® 2000 與 Lipofectamine® 3000  的 2~3 倍。
  • 操作簡易:無須更換培養基並且只需要收集一次病毒液即可獲得高病毒力價,省去濃縮病毒液的步驟與時間。
  • 高安全性:成份不含任何動物來源物質,大幅降低細胞污染的可能性。

 

Up to 100-fold Increase in Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with the pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix Powered by MISSION® or pLenti6.2-GW/EmGFP transfer vector and the ViraPower™ Lentiviral Packaging Mix (1:1 ration, 2 µg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine® 2000 (3:1), or Lipofectamine® 3000 (3:1:1). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

Transfer Vector Selection Greatly Affects Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with one of three transfer vectors: MISSION® pLKO.1-puro-CMV-TurboGFP™, ViraPower™ pLenti6.2-GW/EmGFP, or Precision Lenti-ORF™ RFP and the Lentivirus Packaging Mix powered by MISSION® ) (1:1 ratio, 2 µg/well) using the TransIT®-Lenti Transfection Reagent (3:1, vol:wt). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

 

訂購資訊

Product Pack Size Cat. No.
TransIT® Lentivirus System 5 rxn MR-MIR6650
34 rxn MR-MIR6655

 

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