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找出您實驗的最佳轉染試劑

友善列印Lentivirus Packaging Mix Powered by MISSION®

  • 高生產效率:搭配 TransIT®-Lenti 使用,具感染力的慢病毒 (lentivirus) 力價可高達 108
  • 操作簡易:只需一管即含有組裝 lentivirus 病毒顆粒所需的套膜 (envelop)、蛋白質膜 (capsid) 蛋白元件、蛋以及負責將目標基因嵌入宿主染色體必須的反轉錄脢 (eeverse transcriptase) 和嵌合脢 (integrase)。

 

Up to 100-fold Increase in Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with the pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix Powered by MISSION® or pLenti6.2-GW/EmGFP transfer vector and the ViraPower™ Lentiviral Packaging Mix (1:1 ration, 2 µg/well) with the following reagents: TransIT®-Lenti (3:1, vol:wt), Lipofectamine® 2000 (3:1), or Lipofectamine® 3000 (3:1:1). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

Transfer Vector Selection Greatly Affects Functional Lentivirus Titers. Adherent 293T/17 cells were transfected in a 6-well plate with one of three transfer vectors: MISSION® pLKO.1-puro-CMV-TurboGFP™, ViraPower™ pLenti6.2-GW/EmGFP, or Precision Lenti-ORF™ RFP and the Lentivirus Packaging Mix powered by MISSION® ) (1:1 ratio, 2 µg/well) using the TransIT®-Lenti Transfection Reagent (3:1, vol:wt). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Error bars represent triplicate transfection complexes titered individually. Functional titers were calculated using virus dilutions with less than 20% GFP positive cells.

 

訂購資訊

Product Pack Size Cat. No.
Lentivirus Packaging Mix Powered by MISSION® 5 rxn MR-MIR6630
34 rxn MR-MIR6640

 

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