Revio™ 高精準長讀取定序系統

想要擁有既長又準的基因定序結果，卻擔心定序成本高不可攀嗎？PacBio 公司於 2022 年底全新推出的「Revio 長讀取定序系統」幫助您實現高通量、高效率與高性價比的基因定序，讓您獲得更豐富全面的基因資訊！

高通量：Revio 系統的定序通量比 Sequel IIe 系統提高了 15 倍，相當於每年可完成 1,300 個人類全基因體定序。
高效率：搭載有 Google DeepConsensus，數據運算能力比 Sequel IIe 系統提高 20 倍，能將定序時間縮短至 24 小時完成，同時省下 50% 數據儲存空間與成本。
高性價比：讓基於 HiFi 的全基因體定序推進至 1,000 美元*級別時代。 *以美國定價為例，僅包含試劑耗材成本，不含服務商工本費
靈活簡易：透過簡化的試劑耗材設計、24 小時可完成的定序流程、以及定序期間加載耗材的能力，讓 Revio 系統的運行設置變得更加簡單和靈活。此外，Revio 系統還採用了全新設計的 SMRT 晶片 (SMRT cell)，摒除對外部氮氣供應的需求。

更多詳細資訊歡迎洽詢 PacBio 台灣代理伯森生技。

Revio 系統的定序通量比 Sequel IIe 系統提高了 15 倍，相當於每年可完成 1,300 個人類全基因體定序

	Revio system	Sequel IIe system	Increase
Higher density	25 million ZMW	8 million ZMWM	3×
Independent stages	4	1	4×
Shorter run times	24 hrs	30 hrs	1.25×
30× HiFi human genomes/year	88	1,300	15× overall

Revio 系統具有與 Sequel IIe 系統近似甚至更佳的基因序列變異檢測能力

HiFi sequencing on the Revio system delivers high accuracy for all variants types, matching the precisionFDA-winning accuracy of the Sequel IIe system. Data shown is for a single Revio SMRT Cell for HG002/GM24385.

Revio 系統與其他定序技術平台比較表

	Revio (HiFi sequencing)	SBS sequencing	Nanopore sequencing
Read length	15–20 kb	2x150 bp	10–100 kb
Read accuracy	99.95% (Q33)	99.92% (Q31)	99.26% (Q21)
Run time	24 hours	44 hours	72 hours
Yield	90 Gb	2,400–3,000 Gb	50–110 Gb
Variant calling — SNVs	✓	✓	✓
Variant calling — indels	✓	✓
Variant calling — SVs	✓		✓
5mC methylation	✓		✓
Phasing	✓		✓

產品規格

Sequencing specifications
Library	15–20 kb
Run time	24 hours *Run time specification is for the sequencing reaction.
HiFi yield	1 SMRT Cell: 90 Gb 4 SMRT Cells: 360 Gb *HiFi yield specification based on HG002/GM24385 human DNA extracted with Nanobind CBB kit and prepared with SMRTbell prep kit 3.0. HiFi yield is dependent on library fragment size. Yield is typically lower for shorter libraries.
Base quality	90% Q30+
Methylation	5mC at CpG sites
Application performance
Throughput	Per Revio SMRT Cell Human genome: 1 Single-cell transcriptome: 1 Large gene panel (20 Mb): 12 Per year Human genome: 1,300 Single-cell transcriptome: 1,300 Large gene panel (20 Mb): 15,600
Expected coverage	Human genome: 30× Single-cell transcriptome: 80 million reads Large gene panel (20 Mb): 90% target positions ≥10×
Instrument specifications
Operating environment	Temperature: 19–25°C Humidity: 20–80% relative humidity, non-condensing Ventilation: 13,000 BTU/hr (3,800 W)
Dimensions	W × D × H: 92.7 cm × 91.4 cm × 174.5 cm Weight: 465 kg Floor space: 243.8 cm × 138.5 cm
Electrical power	200–240 VAC at 50-60 Hz, max 5 kVA/4.8 kW/24A
Compute	Network connection: 1 GbE or 10 GbE, copper Output file format: hifi_reads.bam, ≈55 GB each, up to 72 TB per year