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友善列印Label IT® Tracker Intracellular Nucleic Acid Localization Kit

  • 可應用於標定 Plasmid DNA,協助研究者觀察轉染 (transfection) 位置與效率。
  • 化學標定方式,流程簡單,一小時即可完成標定反應。
  • 標定反應不會影響轉染效率。
  • 產品多元彈性,具備多種標定分子、以及包含或不包含轉染試劑的包裝可選。

 

作用原理:

The Label IT® Tracker Kits. The Label IT® Tracker labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with plasmid DNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the plasmid. Attachment of the Label IT® Reagent to plasmids does not alter their structure or affect downstream gene expression, and as such, the same labeled plasmid can be both visualized after transfection by fluorescence microscopy and employed to express the gene of interest on the plasmid.

 

產品效能:


Tracking of Plasmid Localization and Expression. COS-7 cells transfected with Label IT® Tracker Cy™5 (blue) labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Images were acquired at 3, 8, and 20 hours post-transfection. The blue signal indicates the cellular localization of the labeled plasmid, the green signal indicates cellular autofluorescence, and the yellow signal is the expression of the nuclear yellow fluorescent protein (YFP) reporter.
  Tracking of Plasmid Localization. COS-7 cells were transfected with Label IT® Tracker Biotin labeled pEYFP-nuc and TransIT®-LT1 Reagent in serum containing media. Image was acquired at 24 hours post-transfection after staining with streptavidin Cy™3 (red) conjugate. The green signal indicates cellular autofluorescence.

 

訂購資訊

Product Pack Size Cat. No.
含轉染試劑
Label IT® Tracker Intracellular Nucleic Acid Localization Kit, Biotin for 50-200 µg plasmid DNA MR-MIR7024
Label IT® Tracker Intracellular Nucleic Acid Localization Kit, CX-Rhodamine for 50-200 µg plasmid DNA MR-MIR7022
Label IT® Tracker Intracellular Nucleic Acid Localization Kit, Cy™3 for 50-200 µg plasmid DNA MR-MIR7020
Label IT® Tracker Intracellular Nucleic Acid Localization Kit, Cy™5 for 50-200 µg plasmid DNA MR-MIR7021
Label IT® Tracker Intracellular Nucleic Acid Localization Kit, Fluorescein for 50-200 µg plasmid DNA MR-MIR7025
Label IT® Tracker Intracellular Nucleic Acid Localization Kit, TM-Rhodamine for 50-200 µg plasmid DNA MR-MIR7023

 

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