ELISA 免疫反應干擾降低試劑

LowCross-Buffer

• 可大幅降低免疫反應中抗原與中/低親和性抗體產生的結合反應，使得僅有高親和性的抗體可以專一性地結合於抗原上，進而達到減少非專一性結合、降低背景值的效果。

• 使用時僅需先將 LowCross-Buffer 搖晃混和均勻，接著依照平常稀釋抗體的比例直接以 LowCross-Buffer 來稀釋所欲使用的一級或二級抗體即可。

• 適用於 Western Blot、ELISA、EIA、RIA、Protein Array、Immuno-PCR 等實驗。

產品效能：

ELISA: Avoid False-Positive Binding In PBS buffer condition, control of specifity in (A1-12) and blanks (H1-H12) show false positive binding. Protein Array: Reduction of Background Multiple antibodies against an identical analyte spotted on a slide. The signal to noise ratio are: without LowCross-Buffer = 3.42; with LowCross-Buffer = 17.26.

訂購資訊

LowCross-HRP

• 成分內含 "LowCross-Buffer" 與 "HRP 酵素鍵結抗體穩定劑"，兼具有降低實驗背景值、與延長 HRP 酵素鍵結抗體使用壽命的雙重功效。

• 可取代一般 HRP-conjugate Stabilizer 與 HAMA-blocker 試劑，並且具有更佳的效能與便利性！

• 使用時僅需先將 LowCross-HRP 搖晃混和均勻，接著依照平常稀釋 HRP 酵素鍵結抗體的比例，直接以 LowCross-HRP 來稀釋即可。

產品效能：

LowCross-Buffer® reduces interferences in HAMA positive samples to background levels (<40 ng/ml, according to HAMA-ELISA manual). Shelf life of HRP-antibody conjugate is measured as peroxidase signal in an ELISA after binding the analyte. Values are shown as percentage of freshly diluted HRP-antibody conjugate.

訂購資訊

The Blocking Solution

• 內含改良過的高純度 Casein 酪蛋白片段，具有隨取隨用的便利特性以及良好的阻斷效果，可有效降低濾膜或塑膠表面的抗體非專一性結合現象。

• 適用範圍包含 Western Blot、ELISA、RIA、IHC、Protein Array 等實驗。

產品效能：

ELISA (competitive): Decrease of CV (with n=96, measured at the maximum B0) Compared to standard blockers, The Blocking Solution impressively lowered the coefficient of variation (CV) as shown in this successful assay validation of a competitive ELISA.

訂購資訊