µ-Slide Chemotaxis
- 專為 2D 或 3D 細胞趨化性實驗 (chemotaxis assays) 所設計，每片玻片可同時進行 3 個獨立實驗。
- 可穩定維持藥物線性濃度長達 48 小時！
- 可搭配顯微鏡進行即時細胞影像觀察或細胞爬行錄影。
- 懸浮／貼附型細胞、移動快速／移動緩慢細胞皆可適用，例如：癌細胞、淋巴球 (lymphocytes)、嗜中性白血球 (neutrophiles)、單核球 (monocytes) …等。
歡迎與我們聯繫索取更多 µ-Slide Chemotaxis 產品資訊與文獻。
產品特點
操作簡單便利，可輕鬆製造出藥物線性濃度
藥物線性濃度可穩定維持長達 48 小時
Time-stable gradients across the observation area in the µ-Slide Chemotaxis. (A) Top view of the chemotaxis chamber with a collagen I matrix in the observation area. The reservoirs were filled with 50 nM and 1 nM AlexaFluor 488, respectively. Measurements were performed at five different x-positions, indicated in red. (B) Time-stable linear AlexaFluor 488 concentration profiles were created within the migration chamber, measured by fluorescence correlation spectroscopy.
底部厚度符合 #1.5 蓋玻片，影像清晰易觀測
Live cell imaging of actin dynamics in a LifeAct-expressing primary dendritic mouse cell after the application of a chemotactic gradient. For the chemotaxis assay, cells were seeded on the µ-Slide Chemotaxis and a chemotactic gradient (CCL19) was applied. One day after the transfection, F-actin dynamics in the migrating cells were visualized using live cell imaging.
產品規格
| Outer dimensions | W25.5 x L75.5 mm |
| Chemotaxis chambers on slide | 3 |
| Volume per chamber | 130 µl |
| Coating area per chamber when coating full chamber | 3.50 cm² |
| Coating area per chamber when coating observation area only | 0.27 cm² |
| Distance between chambers | 18.5 mm |
| Total height with plugs | 12 mm |
| Volume chemoattractant | 30 µl |
| Bottom | ibidi Polymer Coverslip |
訂購資訊
| Product Name | Surface | Pack Size | Cat. No. |
|---|---|---|---|
| µ-Slide Chemotaxis | ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized | 10 | IB-80326 |
| Collagen IV: #1.5 polymer coverslip, sterilized | 10 | IB-80322 |
