產品介紹產品介紹細胞培養與分析細胞培養耗材細胞培養暨影像觀察兩用玻片/皿µ-Slide Angiogenesis

µ-Slide Angiogenesis

µ-Slide Angiogenesis | ibidi 台灣獨家代理伯森生技

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產品特點

獨特「well-in-a-well」設計可大幅減少每次血管新生實驗所需的細胞、膠體、以及藥物用量,更可提供完美的影像品質

上圖左:「well-in-a-well」設計是指在 µ-Slide Angiogenesis 的每個獨立培養槽內 (直徑 5 mm),還含有一個 4 mm 直徑的槽體可供注入 matrix gel 使用。此內槽僅需 10 µl 的 matrix gel 即可剛好注滿,形成一厚度達 0.8 mm 的平整細胞基質膠層。上圖右:注入在傳統細胞培養盤中的 matrix gel,往往會因為表面張力形成“圓弧形”的膠層,容易導致細胞生長分佈不均的情形,使得可觀察範圍縮小至槽體中央附近;而同樣的表面張力影響性,亦會造成膠層上方所灌注的培養液形成弧形液面,使得所觀察的細胞影像產生扭曲變形的現象。μ-Slide Angiogenesis 玻片的「well-in-a-well」設計完美解決了以上這兩大問題,使得影像可以更清晰且容易觀察,同時大幅減少 matrix gel 的使用量。

底部厚度符合 #1.5 蓋玻片,影像清晰易觀測

(A) Phase contrast image showing one well of the µ-Slide Angiogenesis with HUVEC cells on Matrigel® after 12 hours of incubation during a tube formation assay. Phase contrast (B) and fluorescence microscopy (C) of a single strand composed of HUVEC cells during a tube formation assay in the µ-Slide Angiogenesis. The F-actin cytoskeleton is stained green and the cell nuclei are stained blue.

µ-Plate Angiogenesis 96 Well 具備 µ-Slide Angiogenesis 的一切特質與優點,應用於高通量 (high throughput) 實驗更划算便利

µ-Plate Angiogenesis 96 Well | ibidi 台灣獨家代理伯森生技

產品規格

µ-Slide Angiogenesis 與 µ-Plate Angiogenesis 96 Well 規格比較

  µ-Slide
Angiogenesis
µ-Slide
Angiogenesis
Glass Bottom
µ-Plate
Angiogenesis
96 Well
Outer dimensions W25.5 x L75.5 mm W25.5 x L75.5 mm W85.5 x L127.7 mm
Number of wells 15 15 96
Volume inner well 10 µl 10 µl 10 µ
Ø inner well 4 mm 4 mm 4 mm
Depth inner well 0.8 mm 0.8 mm 0.8 mm
Volume upper well 50 µl 50 µl 70 µl
Ø upper well 5 mm 5 mm 5 mm
Growth area inner well 0.125 cm² 0.125 cm² 0.125 cm²
Coating area using 10 µl 0.23 cm² 0.23 cm² 0.23 cm²
Bottom ibidi Polymer Coverslip Glass Coverslip No. 1.5H ibidi Polymer Coverslip

底部材質規格比較

  #1.5 ibidi Polymer Coverslip
(ibidi 特殊塑料)
#1.5H ibidi Glass Coverslip
(#1.5H 高精度玻璃)
Bottom thickness 180 µm (+10/-5 µm) 170 µm (+/-5 µm)
Bottom material Polymer D 263 M Schott high precision glass
Gas permeability Yes No
Compatibility with protein coatings Yes Yes
Immersion oil compatibility Yes Yes
Autofluorescence Low Low
Transmission Very high (even ultraviolet) High (ultraviolet restrictions)
Refractive index (nD 589 nm) 1.52 1.52
Brightfield Microscopy ++ ++
Phase Contrast ++ ++
Differential Interference Contrast (DIC) ++ ++
Widefield Fluorescence ++ ++
Confocal Fluorescence ++ ++
Total Internal Reflection Fluorescence (TIRF) + ++
Super-Resolution Microscopy + ++

※更多材質特性比較資訊請見這裡

訂購資訊

Product Name Surface Pack Size Cat. No.
µ-Slide Angiogenesis ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 15 IB-81506
Uncoated: #1.5 polymer coverslip, hydrophobic, sterilized 15 IB-81501
µ-Slide Angiogenesis Glass Bottom Glass Bottom: #1.5H (170 µm +/- 5 µm) D 263 M Schott glass, sterilized 15 IB-81507
µ-Plate Angiogenesis 96 Well ibiTreat: #1.5 polymer coverslip, tissue culture treated, sterilized 15 IB-89646
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