Location: Home > Products > Our Products > 細胞分析 > 細胞轉染 > TransIT-VirusGEN® Transfection Reagent

找出您實驗的最佳轉染試劑

友善列印TransIT-VirusGEN® Transfection Reagent

專為提升腺相關病毒(AAV) 與慢病毒 (lentivirus) 產量而設計的細胞轉染試劑。產品特點包含:

  • 生產系統彈性:適用於懸浮型與貼附型 HEK293 細胞生產 AAV 與 lentivirus。
  • 一致性的高病毒產量:維持批次間高效率的病毒產率。
  • 可線性放大產量:即使病毒生產系統放大數十倍,等比例放大轉染條件可維持相同的單位病毒產量。

 

TransIT-VirusGEN® Outperforms Competitor Reagents in Suspension & Adherent AAV Cell Cultures. Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 µg/ml, Agilent Technologies) with the following reagents: TransIT-VirusGEN® (2:1, vol:wt), Lipofectamine® 2000 (3:1, Thermo Fisher), Lipofectamine® 3000 (3:3:1, Thermo Fisher) or 25 kDa PEI (4:1, Polysciences). Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

TransIT-VirusGEN® Outperforms Competitor Reagents in Suspension and Adherent Lentivirus Cell Cultures. Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pLKO.1-puro-CMV-TurboGFP™ transfer vector and the Lentivirus Packaging Mix powered by MISSION® (1:1 ratio, 1 µg/ml) with the following reagents: TransIT-VirusGEN® (3:1, vol:wt), Lipofectamine® 2000 (2:1 (A) or 3:1 (B), Thermo Fisher), Lipofectamine® 3000 (2:2:1 (A) or 3:3:1 (B), Thermo Fisher) or 25 kDa PEI (4:1, Polysciences). The supernatant was harvested, filtered (0.45 µm), and titered using 293T/17 cells. Lentivirus transductions were performed in the presence of 8 µg/ml TransduceIT™ and GFP expression was measured 72 hours post-transduction using guava easyCyte™ 5HT Flow Cytometer. Functional titers were measured from virus dilutions with less than 20% GFP positive cells. The error bars represent the standard deviation of triplicate wells.

TransIT-VirusGEN® Enables Broad Scalability (AAV). Suspension FreeStyle™ 293-F cells grown in FreeStyle™ F17 Medium (A) or adherent 293T/17 cells (B) were transfected with pAAV-hrGFP, pAAV-RC, and pAAV-Helper (1:1:1 ratio, 1.5 total µg/ml, Agilent Technologies) using the TransIT-VirusGEN® Transfection Reagent (2:1 reagent-to-DNA ratio (vol:wt), Mirus) at the indicated volumes perculture vessel. Harvested virus was used to transduce HT1080 cells and GFP expression was measured 48 hours post-transduction by flow cytometry. Functional titers were measured from virus dilutions with less than 20% GFP positive cells.  Error bars represent the standard deviation of triplicate wells, flasks, or dishes.

 

訂購資訊

Product Pack Size Cat. No.
TransIT-VirusGEN® Transfection Reagent 0.3 ml MR-MIR6703
0.75 ml MR-MIR6704
1.5 ml MR-MIR6700
5 x 1.5 ml MR-MIR6705
10 x 1.5 ml MR-MIR6706

 

首頁關於伯森最新消息 產品介紹代客服務聯絡我們 站內搜尋
© BLOSSOM BIOTECHNOLOGIES INC. All rights reserved.