產品介紹細胞培養與分析細胞轉染試劑與電穿孔產品TransIT® Cell Line Specific Transfection Reagents

TransIT® Cell Line Specific Transfection Reagents

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產品效能

Fig. 1 | TransIT®-293 Reagent Achieves High Transfection Efficiency. HEK 293 cells transfected with pEGFP using TransIT®-293 Reagent in complete media for 24 hours.

 

Fig. 2 | TransIT®-BrCa Transfection Reagent Exhibits Higher Luciferase Expression in Breast Cancer Cells Compared to Other Transfection Reagents. Breast Cancer cell lines, MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D, were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 µg of plasmid DNA per well. Luciferase expression was measured at 24 hours post-transfection using a standard assay. Error bars represent the standard deviation of triplicate wells.

 

Fig. 3 | High Efficiency Delivery of Plasmid DNA in Breast Cancer Cell Types. TransIT®-BrCa Transfection Reagent was used to transfect plasmid DNA encoding GFP into MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468 and T47D breast cancer cell lines. Transfections were performed in 35 mm MatTek dishes using 4 µl of TransIT®-BrCa Transfection Reagent to deliver 2 µg of DNA (2:1 reagent:DNA ratio). Images (32X) were captured at 48 hours post-transfection using a Zeiss Axiovert S100 inverted fluorescence microscope.

 

Fig. 4 | TransIT®-Keratinocyte Reagent Achieves High Transfection Efficiency. Near-Diploid Immortalized Keratinocytes transfected with a Beta-galactosidase reporter vector using TransIT®-Keratinocyte Reagent in complete media for 48 hours. Reporter protein was detected using Mirus Bio’s Beta-Galactosidase Staining Kit.

 

Fig. 5 | TransIT®-Insect Outperforms Competitor Transfection Reagents. Insect cell lines (A) Sf9, (B) High Five™, and (C) Drosophila S2 cells were transfected in 96-well plates with 0.1 µg of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagent-to-DNA ratios (µl:µg). Luciferase expression was measured at 48 hours post-transfection. Sf9 and High Five™ cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard error of the mean for triplicate wells.

 

Fig. 6 | Superior Recombinant Protein Expression in High Five™ Cells Using TransIT®-Insect. High Five™ cells were transfected in 6-well plates with 2.5 µg of a GFP expression plasmid driven by an hr5 enhancer/IE1 promoter using the designated reagent at the indicated reagent-to-DNA ratios (µl:µg). Total soluble cell lysates were prepared from cells 72 hours post-transfection. Lysates from 100 µl culture were analyzed by SDS-PAGE and Coomassie blue staining; cells alone (untransfected) is shown as control. Expressed GFP containing 6X His, S, and HSV tags (~38 kDa) was clearly detected in the lysate from the cells that were transfected (*) with the highest level of expression observed at TransIT®-Insect:DNA ratio of 2:1.

 

Fig. 7 | TransIT®-Insect Yields Increased Protein Expression Over Time. Insect cell lines (A) Sf9, (B) High Five™, and (C) Drosophila S2 were transfected in a 96-well plate with 0.1 µg of a luciferase expression plasmid driven by an hr5 enhancer/IE1 promoter using the TransIT®-Insect Transfection Reagent at a reagent-to-DNA ratio of 2:1 (µl:µg). Luciferase expression was measured at three time points, 24, 48 and 72 hours post-transfection. Sf9 and High Five™ cells were cultured and transfected in serum-free media formulations; S2 cells were in serum containing medium. Error bars represent the standard error of the mean for triplicate wells.

訂購資訊

Product Name1 Applicable Cells Efficiency2 Pack Size Cat. No.
TransIT®-293 Transfection Reagent HEK 293, HEK 293T, and related 75–85% 0.4 ml
1.0 ml
5 x 1.0 ml
10 x 1.0 ml
MR-MIR2704
MR-MIR2700
MR-MIR2705
MR-MIR2706
TransIT®-BrCa Transfection Reagent MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-465, T47D 40–80% 0.4 ml
1.0 ml
5 x 1.0 ml
10 x 1.0 ml
MR-MIR5504
MR-MIR5500
MR-MIR5505
MR-MIR5506
TransIT®-CHO Transfection Kit CHO-K1 and related 50–60% 0.4 ml
1.0 ml
5 x 1.0 ml
10 x 1.0 ml
MR-MIR2174
MR-MIR2170
MR-MIR2175
MR-MIR2176
TransIT-HeLaMONSTER® Transfection Kit HeLa and related 50–60% 0.4 ml
1.0 ml
5 x 1.0 ml
10 x 1.0 ml
MR-MIR2904
MR-MIR2900
MR-MIR2905
MR-MIR2906
TransIT®-Jurkat Transfection Reagent Jurkat, Jurkat-E6, RAW 264.7, THP-1, K562, and other lymphoid cell lines 10–25% 0.4 ml
1.0 ml
5 x 1.0 ml
10 x 1.0 ml
MR-MIR2124
MR-MIR2120
MR-MIR2125
MR-MIR2126
TransIT®-Keratinocyte Transfection Reagent NIKS (near diploid immortalized keratinocytes) and primary keratinocytes 20–30% 0.4 ml
1.0 ml
5 x 1.0 ml
10 x 1.0 ml
MR-MIR2804
MR-MIR2800
MR-MIR2805
MR-MIR2806
TransIT®-Insect Transfection Reagentt High Five™, S2, Sf9   0.4 ml
1.0 ml
5 x 1.0 ml
10 x 1.0 ml
MR-MIR6104
MR-MIR6100
MR-MIR6105
MR-MIR6106
  1. Single tube reagents contain the indicated transfection reagent. Transfection reagents with two components are named "Kits".
  2. Transfection efficiency determined by transfection of an EGFP expression vector followed by quantification of the percentage of cells expressing EGFP.
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