受體-配體結合試驗

GPCR 藥物研發領域的技術領導者 – 美商 Revvity 公司，針對 Receptor-Ligand Binding Assay 提供了一系列完整的分析工具，包含：GPCR 受體表現細胞 (cell lines) / 細胞膜 (Membrane prep.)、可供配對作用的 3H / 125I 放射性配體以及 SPA 技術產品等…多元選擇。歡迎您多加利用便利的伯森客戶服務資源 02-2788-2121 / 0800-059-668 / 線上留言，讓我們協助您快速取得適用產品資訊。

Receptor Membranes & NEN® Radioligands
SPA Beads

Receptor Membranes & NEN® Radioligands

請點擊下方【GPCR Receptor】名稱，即可瀏覽其所對應的受體表現細胞 / 細胞膜、與放射性配體產品列表。由於產品新增快速，若您在下表中無法找到您所需要的產品，歡迎洽詢伯森業務專員索取最新產品資訊。

Wild-type Membranes, Parental Cell Lines, Plasmids

5-Hydroxytryptamine

Acetylcholine (Muscarinic)

Corticotropin-Releasing Factor

Gonadotropin-releasing Hormone

Melanin-concentrating Hormone

Prolactin Releasing Peptide

Thyrotropin Releasing hormone

Urotensin

Vasoactive Intestinal Peptide

Vasopressin

SPA Beads

由 SPA (Scintillation Proximity Assay) 鄰近閃爍分析技術所延伸出來的便利產品，微珠本身含有閃爍計數劑成分，具有全均質化的實驗操作步驟，可讓研究者省略麻煩的分離洗滌步驟，還可省下購買閃爍計數液或濾盤的費用。在進行 Receptor-Ligand Binding Assay 時，僅需先將受體或表現有受體的細胞膜鍵結固定在 SPA Beads 表面，當帶有放射性標定的配體與受體結合時，此時由於距離的靠近，使得放射線能量能夠與 SPA Beads 成分中所含有的閃爍計數劑產生能量轉移反應，使放射線轉換為光能，藉由光值的強弱變化即可了解待測藥物對於受體-配體結合的影響性。

依據最終發光訊號波長的不同，可區分為適合以 CCD 影像系統偵測的 SPA Imaging Beads，以及適合以盤式輻射偵測儀偵測的 SPA Scintillation Beads。兩者皆有多種規格可供選擇，歡迎洽詢索取詳細技術資料。

選擇指南：

	SPA Scintillation Beads	SPA Imaging Beads
Instrument	PMT counters (e.g., TopCount™, MicroBeta™)	CCD Imagers
Throughput	Medium to high	High to ultra high
Format	96- or 384-well plates	96-, 384-, 1536-well plates and above
Emission	Blue region (400 nm)	Red region (615 nm)

SPA Imaging Beads 與 SPA Scintillation Beads 分別具有兩種不同組成核心物質可選：

SPA Scintillation Beads	SPA Imaging Beads	Benefit
Polyvinyltoluene (PVT)	Polystyrene (PS)	Easy to dispense and suited to automation and high-throughput screening. Used for the majority of SPA scintillation and SPA imaging applications
Yttrium silicate (YSi)	Yttrium oxide (YOx)	Possess greater scintillation counting efficiency but less suited to automation because their higher density makes them settle more rapidly than PVT or PS beads

訂購資訊 (請點擊下列分類選項，瀏覽詳細訂購資訊)

SPA Scintillation Beads

Core Bead Type	Product	Pack Size	Cat. No.
PVT	PEI Coupled PVT SPA Beads	500 mg	PK-RPNQ0097
	Protein A PVT SPA Beads	500 mg	PK-RPNQ0019
		25 x 500 mg	PK-RPNQ0031
		2 g	PK-RPNQ0069
	WGA Coated PVT SPA Beads	100 mg	PK-RPNQ0252
		500 mg	PK-RPNQ0001
		25 x 500 mg	PK-SPQ0031
		2 g	PK-RPNQ0060
		25 x 2 g	PK-RPNQ0063
	WGA-PEI TYPE A PVT SPA Beads	500 mg	PK-RPNQ0003
		2 g	PK-RPNQ0061
		25 x 2 g	PK-RPNQ0064
	WGA-PEI TYPE B PVT SPA Beads	500 mg	PK-RPNQ0004
		2 g	PK-RPNQ0062
		25 x 2 g	PK-RPNQ0065
YSi	PEI YSi SPA Beads	500 mg	PK-RPQ0745
	Polylysine Coated YSi SPA Beads	1 g	PK-RPNQ0010
	Protein A YSi SPA Beads	500 mg	PK-RPN143
	Protein A YSi SPA Beads	2 g	PK-RPNQ0068
	WGA Coated YSi SPA Beads	250 mg	PK-RPNQ0011
	WGA Coated YSi SPA Beads	1 g	PK-RPNQ0023

SPA Imaging Beads

Core Bead Type	Product	Pack Size	Cat. No.
PS	PEI PS Imaging Beads	50 mg	PK-RPNQ0297
		500 mg	PK-RPNQ0098
		1 g	PK-RPQ0577
	Polylysine PS Imaging Beads	500 mg	PK-RPNQ0294
		2 g	PK-RPQ0786
		25 x 2 g	PK-RPQ0787
	Protein A PS Imaging Beads	500 mg	PK-RPNQ0264
	WGA PS Imaging Beads	50 mg	PK-RPNQ0262
		500 mg	PK-RPNQ0260
		25 x 500 mg	PK-RPNQ0281
		2 g	PK-RPNQ0308
		25 x 2 g	PK-RPNQ0290
	WGA-PEI TYPE A PS Imaging Beads	50 mg	PK-RPNQ0286
	WGA-PEI TYPE A PS Imaging Beads	500 mg	PK-RPNQ0287
	WGA-PEI TYPE B PS Imaging Beads	500 mg	PK-RPNQ0289
YOx	Polylysine YOx Imaging Beads	2 g	PK-RPQ0328
	Protein A YOx Imaging Beads	500 mg	PK-RPNQ0274
	WGA YOx Imaging Beads	50 mg	PK-RPNQ0272
		500 mg	PK-RPNQ0270
		25 x 500 mg	PK-RPNQ0282
		2 g	PK-RPQ0196
		25 x 2 g	PK-RPQ0197
PS & YOx	SPA Imaging Select-a-Bead Kit	6 x 50 mg	PK-RPNQ0291
PS & YOx	SPA Imaging Select-a-Bead Kit	6 x 500 mg	PK-RPNQ0321