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友善列印Label IT® siRNA Tracker Intracellular
Localization Kit

  • 可應用於標定 siRNA,協助研究者觀察 siRNA 於動物細胞或組織內的分佈位置。
  • 化學標定方式,流程簡單,一小時即可完成標定反應。
  • 標定反應不會影響 siRNA 轉染 (transfection) 或抑制 (knockdown) 效率。
  • 產品多元彈性,具備多種標定分子、以及包含或不包含 siRNA 轉染試劑的包裝可選。

 

作用原理:

The Label IT® siRNA Tracker Kits. The Label IT® siRNA Tracker labeling reagents are composed of three regions: the label (a fluorophore or biotin) (green), the linker (yellow) which facilitates electrostatic interactions with the siRNA and miRNA and the reactive alkylating group (blue) that covalently attaches the Label IT® reagents to any reactive heteroatom within the siRNA or miRNA. Attachment of the Label IT® Reagent to siRNA or miRNA does not alter their structure or affect downstream target knockdown performance, and as such, the same labeled siRNAs or miRNAs can be both visualized after transfection by fluorescence microscopy and employed to knockdown target gene expression.

 

產品效能:

Intracellular Localization of labeled siRNA in HeLa cells. HeLa cells were transfected with Label IT® siRNA Tracker Cy™3-labeled siRNA (red) and TransIT-siQUEST® Reagent in complete media for 24 hours. Cells were fixed and counterstained to identify the nuclei (blue) and the actin (green).
Labeling of siRNA with Label IT® siRNA Tracker Does Not Affect Functionality. TransIT-TKO® Transfection Reagent was used to transfect anti-firefly luciferase siRNA into CHO-luc cells that stably express firefly luciferase. The siRNA was either unlabeled or labeled with Label IT® siRNA Tracker Cy™3, Cy™5, Fluorescein, or CX-Rhodamine Reagents. Bars indicate the percent firefly luciferase expression 24 hours after delivery of 5 nM antifirefly luciferase siRNA.
Delivery of Labeled siRNA to Mouse Liver. siRNA (25 µg) was labeled with Label IT® siRNA Tracker Cy™3 Kit and delivered to mice through the tail vein using either low volume injection conditions (100 µl)(left) or hydrodynamic injection conditions (2 ml over 6-8 seconds) (right). Mouse livers were harvested 30 minutes after injection, fixed in paraformaldehyde, sectioned (10 µm thick) and analyzed. A representative hepatocyte (h) and sinusoid (s) are indicated in each panel. The hydrodynamic injection of siRNA results in siRNA uptake by the majority of the hepatocytes in the liver.

 

訂購資訊

Product Pack Size Cat. No.
Label IT® siRNA Tracker Intracellular Localization Kit, Biotin for 50 µg siRNA MR-MIR7217
Label IT® siRNA Tracker Intracellular Localization Kit, CX-Rhodamine for 50 µg siRNA MR-MIR7214
Label IT® siRNA Tracker Intracellular Localization Kit, Cy™3 for 50 µg siRNA MR-MIR7212
Label IT® siRNA Tracker Intracellular Localization Kit, Cy™5 for 50 µg siRNA MR-MIR7213
Label IT® siRNA Tracker Intracellular Localization Kit, Fluorescein for 50 µg siRNA MR-MIR7216
Label IT® siRNA Tracker Intracellular Localization Kit, TM-Rhodamine for 50 µg siRNA MR-MIR7215

 

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