GTP 結合試驗 (GTP Binding Assay)

Receptor Membranes

請點擊下方【GPCR Receptor】名稱，即可瀏覽其所對應的受體表現細胞 / 細胞膜、與放射性配體產品列表。由於產品新增快速，若您在下表中無法找到您所需要的產品，歡迎洽詢伯森業務專員索取最新產品資訊。

Wild-type Membranes

Wild-type Control Membranes Product Product Type Host Cell Cat. No. CHO-K1 Cells Membrane prep. CHO-K1 PK-RBCCHOM010MG

5-Hydroxytryptamine

5-Hydroxytryptamine Subtype Product Type Host Cell Cat. No. Complementary Ligands 5-HT1A ValiScreen CHO-K1 PK-ES-310-C PK-NET638

Acetylcholine (Muscarinic)

Acetylcholine (Muscarinic) Subtype Product Type Host Cell Cat. No. Complementary Ligands M2 ValiScreen CHO-K1 PK-ES-211-C PK-NET656 M4 ValiScreen CHO-K1 PK-ES-213-C PK-NET656

Adenosine

Adenosine Subtype Product Type Host Cell Cat. No. Complementary Ligands A1 ValiScreen CHO-K1 PK-ES-010-C PK-NET811 Membrane prep. CHO-K1 PK-ES-010-M400UA   A3 ValiScreen CHO-K1 PK-ES-012-C   Membrane prep. CHO-K1 PK-ES-012-M400UA

Adrenoceptors

Adrenoceptors Subtype Product Type Host Cell Cat. No. Complementary Ligands α2A ValiScreen MCHO-K1 PK-ES-030-C PK-NEX253 Membrane prep. CHO-K1 PK-ES-030-M400UA PK-NET613 α2B ValiScreen CHO-K1 PK-ES-031-C PK-NEX253 Membrane prep. CHO-K1 PK-ES-031-M400UA PK-NET613, PK-NET623, PK-NET659, PK-NET678, PK-NET722, PK-NET853

Anaphylatoxin

Anaphylatoxin Subtype Product Type Host Cell Cat. No. Complementary Ligands C3a ValiScreen CHO-K1 PK-ES-730-C   C5a ValiScreen CHO-K1 PK-ES-731-C

Apelin

Apelin Subtype Product Type Host Cell Cat. No. Complementary Ligands APJ ValiScreen CHO-K1 PK-ES-460-C   Membrane prep. CHO-K1 PK-ES-460-M400UA

Cannabinoid

Cannabinoid Subtype Product Type Host Cell Cat. No. Complementary Ligands CB1 ValiScreen CHO-K1 PK-ES-110-C PK-NET1058 Membrane prep. CHO-K1 PK-ES-110-M400UA PK-NET1073 CB2 ValiScreen CHO-K1 PK-ES-111-C PK-NET1051 Membrane prep. CHO-K1 PK-ES-111-M400UA

Chemokine

Chemokine Subtype Product Type Host Cell Cat. No. Complementary Ligands CCR2b ValiScreen CHO-K1 PK-ES-133-C   Membrane prep. CHO-K1 PK-ES-133-M400UA   CCR6 ValiScreen CHO-K1 PK-ES-139-C   Membrane prep. CHO-K1 PK-ES-139-M400UA   CCR7 ValiScreen CHO-K1 PK-ES-140-C   Membrane prep. CHO-K1 PK-ES-140-M400UA   CCR8 ValiScreen CHO-K1 PK-ES-136-C   Membrane prep. CHO-K1 PK-ES-136-M400UA   CX3CR1 ValiScreen CHO-K1 PK-ES-137-C   Membrane prep. CHO-K1 PK-ES-137-M400UA   CXCR2 ValiScreen CHO-K1 PK-ES-145-C   Membrane prep. CHO-K1 PK-ES-145-M400UA   CXCR3 ValiScreen CHO-K1 PK-ES-142-C   Membrane prep. CHO-K1 PK-ES-142-M400UA   CXCR6 ValiScreen CHO-K1 PK-ES-720-C   Membrane prep. CHO-K1 PK-ES-720-M400UA   XCR1 ValiScreen CHO-K1 PK-ES-148-C   Membrane prep. CHO-K1 PK-ES-148-M400UA

Dopamine

Dopamine Subtype Product Type Host Cell Cat. No. Complementary Ligands D3 ValiScreen CHO-K1 PK-ES-173-C PK-NET673 Membrane prep. CHO-K1 PK-ES-173-M400UA PK-NET131

Formyl Peptide

Formyl Peptide Subtype Product Type Host Cell Cat. No. Complementary Ligands FPR2 (FPRL1) ValiScreen CHO-K1 PK-ES-610-C   Membrane prep. CHO-K1 PK-ES-610-M400UA

GABAB

GABAB Subtype Product Type Host Cell Cat. No. Complementary Ligands GABAB1a/B2 ValiScreen CHO-K1 PK-ES-500-C   Membrane prep. CHO-K1 PK-ES-500-M400UA

Galanin

Galanin Subtype Product Type Host Cell Cat. No. Complementary Ligands GAL1 ValiScreen CHO-K1 PK-ES-510-C PK-NEX333 Membrane prep. CHO-K1 PK-ES-510-M400UA

Histamine

Histamine Subtype Product Type Host Cell Cat. No. Complementary Ligands H3 ValiScreen CHO-K1 PK-ES-392-C   Membrane prep. CHO-K1 PK-ES-392-M400UA   H4 ValiScreen CHO-K1 PK-ES-393-C   Membrane prep. CHO-K1 PK-ES-393-M400UA

Leukotriene

Leukotriene Subtype Product Type Host Cell Cat. No. Complementary Ligands BLT1 (LTB4R1) ValiScreen CHO-K1 PK-ES-340-C   Membrane prep. CHO-K1 PK-ES-340-M400UA

Melanin-concentrating Hormone

Melanin-concentrating Hormone Subtype Product Type Host Cell Cat. No. Complementary Ligands MCH1 ValiScreen CHO-K1 PK-ES-370-C PK-NEX375 Membrane prep. CHO-K1 PK-ES-370-M400UA PK-NEX406

Melatonin

Melatonin Subtype Product Type Host Cell Cat. No. Complementary Ligands MT1 ValiScreen CHO-K1 PK-ES-620-C   Membrane prep. CHO-K1 PK-ES-620-M400UA   MT2 ValiScreen CHO-K1 PK-ES-621-C   Membrane prep. CHO-K1 PK-ES-621-M400UA

Neuropeptide FF

Neuropeptide FF Subtype Product Type Host Cell Cat. No. Complementary Ligands NPFF2 ValiScreen CHO-K1 PK-ES-490-C

Nicotinic

Nicotinic Subtype Product Type Host Cell Cat. No. Complementary Ligands GPR109A (HM74A) ValiScreen CHO-K1 PK-ES-760-C   Membrane prep. CHO-K1 PK-ES-760-M400UA

Opioid

Opioid Subtype Product Type Host Cell Cat. No. Complementary Ligands kappa ValiScreen CHO-K1 PK-ES-541-C PK-NET952 Membrane prep. CHO-K1 PK-ES-541-M400UA   mu ValiScreen CHO-K1 PK-ES-542-C PK-NET719 Membrane prep. CHO-K1 PK-ES-542-M400UA PK-NET902 NOP (ORL1) ValiScreen CHO-K1 PK-ES-230-C PK-NET1130

Prostanoid

Prostanoid Subtype Product Type Host Cell Cat. No. Complementary Ligands DP2 (CRTH2) ValiScreen CHO-K1 PK-ES-561-C PK-NET428 Membrane prep. CHO-K1 PK-ES-561-M400UA

Somatostatin

Somatostatin Subtype Product Type Host Cell Cat. No. Complementary Ligands sst1 ValiScreen CHO-K1 PK-ES-520-C PK-NEX389 Membrane prep. CHO-K1 PK-ES-520-M400UA   sst2a ValiScreen CHO-K1 PK-ES-521-C   Membrane prep. CHO-K1 PK-ES-521-M400UA   sst3 ValiScreen CHO-K1 PK-ES-523-C   Membrane prep. CHO-K1 PK-ES-523-M400UA   sst4 ValiScreen CHO-K1 PK-ES-524-C   Membrane prep. CHO-K1 PK-ES-524-M400UA   sst5 ValiScreen CHO-K1 PK-ES-522-C   Membrane prep. CHO-K1 PK-ES-522-M400UA

SPA Beads

由 SPA (Scintillation Proximity Assay) 鄰近閃爍分析技術所延伸出來的便利產品，微珠本身含有閃爍計數劑成分，具有全均質化的實驗操作步驟，可讓研究者省略麻煩的分離洗滌步驟，還可省下購買閃爍計數液或濾盤的費用。在進行 GTP Binding Assay 時，僅需先將受體或表現有受體的細胞膜鍵結固定在SPA Beads 表面，當帶有放射性標定的 GTP 與受體結合時，此時由於距離的靠近，使得放射線能量能夠與 SPA Beads 成分中所含有的閃爍計數劑產生能量轉移反應，使放射線轉換為光能，藉由光值的強弱變化即可了解待測藥物對於 GTP 與受體結合的影響性。

依據最終發光訊號波長的不同，可區分為適合以 CCD 影像系統偵測的 SPA Imaging Beads，以及適合以盤式輻射偵測儀偵測的 SPA Scintillation Beads。兩者皆有多種規格可供選擇，歡迎洽詢索取詳細技術資料。

選擇指南：

SPA Imaging Beads 與 SPA Scintillation Beads 分別具有兩種不同組成核心物質可選：

訂購資訊 (請點擊下列分類選項，瀏覽詳細訂購資訊)

SPA Scintillation Beads

Core Bead Type Product Pack Size Cat. No. PVT WGA Coated PVT SPA Beads 100 mg PK-RPNQ0252 500 mg PK-RPNQ0001 25 x 500 mg PK-SPQ0031 2 g PK-RPNQ0060 25 x 2 g PK-RPNQ0063 YSi WGA Coated YSi SPA Beads 250 mg PK-RPNQ0011 1 g PK-RPNQ0023

SPA Imaging Beads

Core Bead Type Product Pack Size Cat. No. PS WGA PS Imaging Beads 50 mg PK-RPNQ0262 500 mg PK-RPNQ0260 25 x 500 mg PK-RPNQ0281 2 g PK-RPNQ0308 25 x 2 g PK-RPNQ0290 YOx WGA YOx Imaging Beads 50 mg PK-RPNQ0272 500 mg PK-RPNQ0270 25 x 500 mg PK-RPNQ0282 2 g PK-RPQ0196 25 x 2 g PK-RPQ0197

GTP Gamma S GPCR Assay

由 SPA (Scintillation Proximity Assay) 鄰近閃爍分析技術所延伸出來的便利產品，內含 WGA PVT SPA Beads 與 5X Assay Buffer，可供建立全均質化的 GTP 結合分析試驗。偵測原理是利用 SPA Beads 表面所帶有的 WGA 分子將受體表現細胞膜 (membrane prep.) 結合於微珠表面，當帶有放射性標定的 GTP 與受體結合時，此時由於距離的靠近，使得放射線能量能夠與 SPA Beads 成分中所含有的閃爍計數劑產生能量轉移反應，使放射線轉換為光能，藉由光值的強弱變化即可了解待測藥物對於 GTP 與受體結合的影響性。

訂購資訊