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友善列印Express Cloning Checker Kit

由美商 BioChain 公司所獨家研發的便利試劑,可讓傳統的基因選殖確認步驟 (確認所挑選的 colony 內是否含有 plasmid、確認 plasmid 內是否嵌有所要的 gene insert) 縮短至 1~2 小時即可完成,不但大幅縮短確認時程,還能同時進行大量樣本的檢測確認步驟,讓基因選殖實驗更有效率!

  • Express Cloning Checker Kit I:此產品可讓實驗者不需經過麻煩的「菌液培養→ plasmid 萃取」步驟,即可直接檢測培養盤中的 colony 是否已成功轉殖入 plasmid。實驗流程簡單快速,只需挑取部分 colony 加入 A 劑與 B 劑,均勻混和後跑電泳,即可由電泳結果立刻確認 plasmid DNA 存在與否。藉由本產品,實驗者可於半個工作天內迅速檢測多達上百個 colonies,同時亦可應用於 glycerol stock 菌液或新鮮菌液的確認檢測,隨貨並附 Supercoiled DNA Marker (size: 3, 3.9, 5.4, 7, 10 kb) 方便結果判讀。

  • Express Cloning Checker Kit II:此產品可讓實驗者不需經過麻煩的「plasmid 萃取」步驟,即可直接以 colony 或菌液進行限制脢切割反應,與傳統需耗時兩天的流程相比 (菌液培養→ plasmid 萃取→ 限制脢切割),搭配 Express Cloning Checker Kit I 使用,可讓整個基因選殖確認步驟 (確認 colony 是否含有 plasmid → 確認 plasmid 是否含有 insert) 縮短至 1~2 小時即可完成,大幅提高實驗效率。

  • Express Cloning Checker Kit III:I & II 組合包。

 


Express Cloning Checker Kit can shorten the operation procedure by direct analyzing plasmid recombinants in bacteria without extracting plasmid DNA.
 (A). Partial colonies were picked up directly from over-night cultured transformation plates and treated withred and yellow solutions before electrophoresis.Three recombinants with 2.5 kb insert (lanes 3, 5 and8) are identified. Lane M is the supercoiled DNA marker supplied in the kit. (B). Recombinant colonies were picked up directly from overnight cultured transformation plates in green solution and followed by restriction enzyme digestion (EcoR I lanes 1 and 2) for 30 minutes at 37°C. Lane M is Hind III/lambda DNA marker. An 838 bp insert was detected.

 

訂購資訊

Product Pack Size Cat. No.
Express Cloning Checker Kit I 200 reactions BC-K5011200
Express Cloning Checker Kit II 200 reactions BC-K5012100
Express Cloning Checker Kit III 200 reactions BC-K5013200

 

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