產品介紹表觀遺傳學研究DNA 甲基化分析試劑 (DNA Methylation Analysis Kits and Reagents)

DNA 甲基化分析試劑

目前已知異常的 DNA 甲基化程度與多種癌症和疾病具有高度相關性,因此可藉由找尋調控此機制的方式,開發具有潛力的治療藥物。以下我們將為您介紹針對偵測 Genomic DNA 甲基化程度所設計的便利試劑組「DNA Methylation Detection Kit」,以及各種相關抗體與抑制劑產品。同時我們也將介紹如何利用 Revvity 公司特殊研發的 Alpha 技術建立起全均質化的快速分析平台。歡迎與我們聯繫索取完整產品資訊與優惠活動訊息。

DNA Methylation Detection Kit

BioChain 公司所開發的「DNA Methylation Detection Kit」是針對偵測 Genomic DNA 甲基化程度所設計的便利試劑組,可於三小時內完成亞硫酸鹽處理步驟。

亞硫酸鹽 (bisulfite, NaHSO3) 具有能夠將 CpG 裡的 Cytosine 轉變為 Uracil(使得原本兩股互補的 DNA 序列形成無法互補配對的單股序列),以及將 mCpG 裡的 5-Methyl Cytosine 轉變為 Cytosine 的特性(可維持原本兩股互補配對狀態)。經亞硫酸鹽處理過的 Genomic DNA 樣本可透過甲基化專一性 PCR (Methylation Specific PCR, MSP)、定序或微陣列晶片…等方式,測得 Genomic DNA 的甲基化程度。

產品特點

訂購資訊

Product Name Pack Size Cat. No.
DNA Methylation Detection Kit 50 rxn BC-K5082100

Antibodies, Inhibitors

Enzo Life Sciences 公司針對 Epigenetics 研究推出了一系列完整的分析工具,以下即為您摘錄 DNA Methylation 相關研究試劑。由於品項眾多無法一一詳列,歡迎洽詢伯森業務專員索取完整產品資訊。

Antibodies

Product Name Reactivity Application Cat. No.
Dnmt1 monoclonal antibody (60B1220.1) H, M, Z ICC, IHC, IP, WB ALX-804-369
Dnmt3a (mouse) monoclonal antibody (64B1446) M ICC, IHC, IP, WB ALX-804-370

H = Human, ICC = Immunocytochemistry, IHC = Immunohistochemistry, IP = Immunoprecipitation, M = Mouse, WB = Western Blot, Z = Zebrafish

Inhibitors

Product Name Description Cat. No.
5-Aza-2'-deoxycytidine DNA methyltransferase inhibitor ALX-480-096
(-)-Epigallocatechin gallate Inhibitor of NOS, telomerase and Dnmt ALX-270-263
Zebularine Dnmt inhibitor BML-GR344
SCREEN-WELL® Epigenetics library The SCREEN-WELL® Epigenetics library contains 43 inhibitors of these important enzymes: HDACs, SIRTs, Lysine demethylases, HATs, Histone methyl transferases, DNA methyltransferases as well as SIRT activators BML-2836

Alpha for DNA Methylation Assays

「Alpha Toolbox Beads」系列是由 Alpha 技術所延伸出來的便利產品,能夠自由組合出多種偵測平台,以取代步驟繁複的 PCR、EMSA、ChIP、ELISA 等類型產品,讓研究者僅需使用單一技術就能進行 DNA methylation、DNA:Protein interaction、Histone modification 等多項表觀遺傳學分析實驗。以下我們就以 DNA Methyltransferase 1 (DNMT1) 酵素活性分析實驗為例,介紹如何利用 Alpha 技術建立起簡單快速的分析平台。

Alpha DNMT1 Assay 偵測原理與範例數據

Alpha 技術主要包含有兩個核心試劑,分別是 Donor bead 與 Acceptor bead。在進行反應時,僅需藉由特定的分子結合將兩者拉近,即可在 680 nm 雷射光激發下發出 615 nm (AlphaLISA acceptor bead) 或 520-620 nm (AlphaScreen acceptor bead) 的螢光訊號。以 DNMT1 酵素活性分析實驗來說,準備下列試劑即可進行實驗。

 

Alpha 技術不僅流程簡單快速,還能提供精確量化的實驗分析結果。分別在 30 µM SAM 與 600 µM SAM 濃度條件下,使用已知的 DNMT1 活性促進劑 "DMSO",以及已知的 DNMT1 抑制劑 "Sinefungin" 和 "SAH",測試 Alpha 平台的偵測能力。結果證實 Alpha 技術的確能夠專一且準確地進行 DNMT1 酵素活性偵測。

Alpha Toolbox Beads

只要掌握 Alpha 技術的偵測原理,您就可以透過 Alpha Toolbox Beads 系列產品自由組合出各種偵測平台,應對各種不同實驗需求與生物樣本特性。以下為您整理出各種相關產品的訂購資訊,歡迎與我們聯繫取得適用產品建議與完整技術支援。

Single Beads

Bead Coating Alpha Donor Beads AlphaLISA Acceptor Beads AlphaScreen Acceptor Beads
Anti-bovine IgA PK-AL169
Anti-bovine IgG PK-AL165
Anti-bovine IgG1 PK-AL166
Anti-bovine IgM PK-AL168
Anti-bovine-IgG2 PK-AL167
Anti-chicken IgY PK-AL131
Anti-c-myc PK-AL111
Anti-DIG PK-AS108 PK-AL113
Anti-DNP PK-AS111 PK-AL173
Anti-FITC PK-AS115 PK-AL127
Anti-FLAG PK-AS103 PK-AL112
Anti-GFP PK-AS112 PK-AL133
Anti-goat IgG PK-AL107
Anti-GST PK-AL110
Anti-HA PK-AL170
Anti-His PK-AL128
Anti-HRP PK-AS109 PK-AL171
Anti-human IgG (Fc-specific)  PK-AS114 PK-AL103
Anti-human IgG1 PK-AL141
Anti-human IgG1 (isotyping) PK-AL153
Anti-human IgG2 (isotyping) PK-AL154
Anti-human IgG4 PK-AL142
Anti-human IgG4 (isotyping) PK-AL156
Anti-maltose binding protein (MBP) PK-AL134
Anti-mouse IgE (isotyping) PK-AL161
Anti-mouse IgG PK-AS104 PK-AL164
Anti-mouse IgG (Fc-specific) PK-AL105
Anti-mouse IgG1 (isotyping) PK-AL157
Anti-mouse IgG2a (isotyping) PK-AL158
Anti-mouse IgG2b (isotyping) PK-AL159
Anti-mouse IgG3 (isotyping) PK-AL160
Anti-mouse IgM PK-AL130
Anti-mouse IgM (isotyping) PK-AL162
Anti-Protein C Tag PK-AL172
Anti-rabbit IgG PK-AS105 PK-AL104
Anti-rat IgG PK-AS110 PK-AL106
Anti-sheep IgG PK-AL132
Anti-V5 PK-AL129
Glutathione PK-6765300 PK-AL109
Human IgG (Fc-fragment) PK-AS113 PK-AL174
Human IgG (whole) PK-AL175
Lens culinaris agglutinin (LCA) PK-AL140
Ni chelate PK-AS101 PK-AL108
Protein A PK-AS102 PK-AL101 PK-6760137
Protein G PK-AL102
Protein L PK-AL126
Strep-Tactin PK-AS106 PK-AL136
Streptavidin PK-6760002 PK-AL125
Unconjugated PK-6762013 PK-6772001 PK-6762003

AlphaScreen Bead Sets

Product Name Donor Bead Acceptor Bead Size
(Assay Points in 384-well Format)
Cat. No.
AlphaScreen IgG (Protein A) Detection Kit Streptavidin Protein A 500 PK-6760617C
10,000 PK-6760617M
50,000 PK-6760617R
AlphaScreen Mouse IgG Detection Kit Streptavidin Anti-mouse IgG 500 PK-6760606C
10,000 PK-6760606M
50,000 PK-6760606R
AlphaScreen Rabbit IgG Detection kit Streptavidin Anti-rabbit IgG 500 PK-6760607C
10,000 PK-6760607M
50,000 PK-6760607R
AlphaScreen anti-phosphotyrosine (PY20) kit Streptavidin Anti-Phosphotyrosine (PY20) 500 PK-6760601C
10,000 PK-6760601M
50,000 PK-6760601R
AlphaScreen anti-phosphotyrosine (P-Tyr-100) kit Streptavidin Anti-Phosphotyrosine (P-Tyr-100) 500 PK-6760620C
10,000 PK-6760620M
50,000 PK-6760620R
AlphaScreen anti-phosphotyrosine (PT66) kit Streptavidin Anti-Phosphotyrosine (PT66) 500 PK-6760602C
10,000 PK-6760602M
50,000 PK-6760602R
AlphScreen Antibody-Free PhosphoSensor Kit Streptavidin Phosphosensor (Lewis metal) 1,000 PK-6760307D
10,000 PK-6760307M
50,000 PK-6760307R
AlphaScreen c-myc Detection Kit Streptavidin Anti-c-myc 500 PK-6760611C
10,000 PK-6760611M
50,000 PK-6760611R
AlphaScreen DIG (Digoxin/Digoxigenin) Detection Kit Streptavidin Anti-DIG 500 PK-6760604C
10,000 PK-6760604M
50,000 PK-6760604R
AlphaScreen FITC (Fluorescein) Detection Kit Streptavidin Anti-FITC (Fluorescein) 500 PK-6760605C
10,000 PK-6760605M
50,000 PK-6760605R
AlphaScreen FLAG (M2) Detection Kit Streptavidin Anti-FLAG 500 PK-6760613C
10,000 PK-6760613M
50,000 PK-6760613R
AlphaScreen GST Detection Kit Streptavidin Anti-GST 500 PK-6760603C
10,000 PK-6760603M
50,000 PK-6760603R
AlphaScreen HA (Hemagglutinin) Detection Kit Streptavidin Anti-HA 500 PK-6760612C
10,000 PK-6760612M
50,000 PK-6760612R
AlphaScreen Histidine (Nickel chelate) Detection Kit Streptavidin Nickel chelate 500 PK-6760619C
10,000 PK-6760619M
50,000 PK-6760619R

Other Alpha Products

Classification Product Pack Size Cat. No.
Buffers AlphaLISA Bovine Immunoassay Buffer (10X) 10 ml PK-AL010C
100 ml PK-AL010F
AlphaLISA Casein Buffer, 5X 10 ml PK-AL014C
100 ml PK-AL014F
AlphaLISA Dissociation Buffer, 3X 10 ml PK-AL006C
100 ml PK-AL006F
AlphaLISA Epigenetics Buffer 1, 5X 10 ml PK-AL008C
100 ml PK-AL008F
AlphaLISA HiBlock Buffer, 10X 10 ml PK-AL004C
100 ml PK-AL004F
AlphaLISA Immunoassay Buffer, 10X 10 ml PK-AL000C
100 ml PK-AL000F
AlphaLISA Lysis Buffer, 5X 10 ml PK-AL003C
100 ml PK-AL003F
AlphaLISA NaCl Buffer, 5X 10 ml PK-AL007C
100 ml PK-AL007F
AlphaLISA PPI Buffer, 5X 10 ml PK-AL015C
100 ml PK-AL015F
AlphaLISA Universal Buffer, 5X 10 ml PK-AL001C
100 ml PK-AL001F
Bead Conjugation Kit AlphaScreen Conjugation Kit
Bead set containing streptavidin-coated Alpha Donor beads and unconjugated AlphaScreen Acceptor beads for creating your own Alpha no-wash assay. Unconjugated beads are amine-reactive, and can be used to create beads conjugated to your own biomolecule-of-interest.
2 x 2 mg PK-6760000K
Controls AlphaScreen Omnibeads
Single bead instrument control that contains the chemistry of both Alpha Donor beads and AlphaScreen Acceptor beads.
1,000 assay PK-6760626D
10,00 assay PK-6760626M
50,00 assay PK-6760626R
AlphaLISA TruHits Kit
Control beads for identifying false positives and interfering compounds in AlphaLISA no-wash assays.
1,000 assay PK-AL900
AlphaScreen TruHits Kit
Bead control set for identifying false positives and interfering compounds.
1,000 assay PK-6760627D
10,00 assay PK-6760627M
AlphaScreen Biotinylated-GST
Biotinylated-GST, to be used as a probe, assay control, or QC control in Alpha (AlphaLISA, AlphaScreen, or AlphaPlex) no-wash assays.
1.5 ml PK-6760305M
AlphaScreen Biotinylated-HIS
Biotinylated-His peptide, to be used as a probe, assay control, or QC control in Alpha (AlphaLISA, AlphaScreen, or AlphaPlex) no-wash assays. This product contains 0.5 mL of biotinylated-His at 5 µM. This probe can be used with either Nickel chelate beads or Anti-His antibody-coated beads.
500 μl PK-6760303M
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