Cellufine™ ET clean

Protein 細胞轉染或動物實驗效率不佳？推薦採用 Cellufine™ ET clean 去除內毒素污染 | JNC Corporation 台灣代理伯森生技內毒素 (endotoxin) 是革蘭氏陰性菌細胞壁外膜所含有的脂多醣體 (lipopolysaccharide, LPS)，在細菌解體死亡 (lysis) 時會被釋放出來，具潛在毒性，在自細菌純化重組蛋白的實驗中，是常見的汙染源。單單使用加熱或是吸附的方式，難以有效去除內毒素。伯森特別推薦日本百年大廠 JNC Corporation 研發製造的「Cellufine™ ET clean」親和性層析膠體，攜帶有修飾過的 poly(ε-lysine) 基團，與內毒素具有高結合力與高專一性，能有效移除樣本中的內毒素汙染，使樣本適用於細胞轉染或動物注射等下游實驗。

產品特點

高結合力：對內毒素的結合力高達 500–1,000 μg/ml，能去除 >99% 的內毒素。
高清除率：具有絕佳清除效果，處理後內毒素濃度 <0.1 EU/ml。
高穩定性：可重覆使用 5 次以上，且不影響效能。
高樣本回收率：可保持 ≥95% 的蛋白質樣本回收率。

歡迎與我們聯繫索取更多 Cellufine™ ET clean 產品資訊與文獻。

產品效能

	Cellufine™ ET clean L		Cellufine™ ET clean S
	μ = 0.4, pH 7.0		μ = 0.05, pH 7.0
	Concentration of endotoxin after treatment (pg/ml)	Recovery of protein after treatment (%)	Concentration of endotoxin after treatment (pg/ml)	Recovery of protein after treatment (%)
Ovalbumin pI = 4.6 Concentration of endotoxin before treatment = 28,000 pg/ml	<10	95	81	99
BSA pI = 4.9 Concentration of endotoxin before treatment = 32,000 pg/ml	<10	97	45	99
Myoglobin pI = 6.8 Concentration of endotoxin before treatment = 4,500 pg/ml	<10	98	18	99
γ-globulin pI = 7.4 Concentration of endotoxin before treatment = 5,600 pg/ml	<10	97	20	99
Cytochrome C pI = 10.6 Concentration of endotoxin before treatment = 1,500 pg/ml	<10	98	15	99

Table 1 | Selective removal of endotoxin from a protein solution by Cellufine™ ET clean beads. The removal of endotoxin was determined by a batchwise method with 0.3 ml of wet adsorbent and 2 ml of a protein solution (1 mg/ml) containing natural endotoxin.

Fig. 1 | Selective adsorption of endotoxin (LPS) from a bovine serum albumin (BSA) solution by Cellufine™ ET clean beads. Selective adsorption of endotoxin was determined using a batchwise method with 0.2 g of the wet beads and 2 ml of a sample solution (BSA: 500 μg/ml, E. coli O111: B4 LPS: 100 ng/ml, pH 7.0, ionic strength of μ = 0.05-0.8).

Fig. 2 | Removal of the endotoxin from Insulin chain A by Cellufine™ ET clean L. Injection sample: 13 mg/ml, 309 EU/ml, 1 ml.

產品規格

	Cellufine™ ET clean L	Cellufine™ ET clean S
Pore Size*	>M_lim 2 x 10⁶	M_lim 2 x 10³
Wet Bead Diameter	ca. 40-130 μm	ca. 40-130 μm
Supplied	a slurry in 20 % ethanol	a slurry in 20 % ethanol

*The pore size (molecular weight exclution; M_lim) of the beads was estimated from calibration curves obtained by size exclusion chromatography. Pullulan and maltose were used for the M_lim determination.

訂購資訊

Product Name	Pack Size	Cat. No.
Cellufine ET clean L	10 ml	CHI-681984324
	50 ml	CHI-681984326
	500 ml	CHI-681984328
	5 L	CHI-681984330
	10 L	CHI-681984335
	1 ml x 5 (Mini-Column)	CHI-20051
	5 ml x 1 (Mini-Column)	CHI-20015
Cellufine ET clean S	10 ml	CHI-682985324
	50 ml	CHI-682985326
	500 ml	CHI-682985328
	5 L	CHI-682985330
	10 L	CHI-682985335
	1 ml x 5 (Mini-Column)	CHI-20151
	5 ml x 1 (Mini-Column)	CHI-20115