Cellometer Auto T4 明視野自動細胞計數儀
- 讀取快速：30 秒內完成細胞計數、影像擷取，以及細胞濃度、直徑、存活度分析。
- 結果精確：專利細胞辨識技術，即使是聚集細胞、不規則形狀細胞皆能準確計數，與傳統 Hemocytometer 相比變異係數 (CV) 更低。
- 符合規範：可依需求提供 IQ/OQ Validation 以及 GMP/GLP software。
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Fig. 1 | Simple Cell Counting & Trypan Blue Viability in Seconds! Within 30 seconds, the Auto T4 instrument reports: • Live, dead, and total cell count • Live and total cell concentration • Mean Cell Diameter • % Viability.
Fig. 2 | Analyze >99% of Cultured Mammalian Cells, including NCI-60 and clumpy MCF-7 cells. (A) The Cellometer cell roundness setting can be adjusted for recognition and counting of irregular-shaped cells, such as RD cells and activated T-cells. (B) The MCF-7 breast cancer cell line can be very clumpy. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis.
Fig. 3 | The Auto T4 Cell Counter Automatically generates a cell size histogram based on cell diameter. ecause Cellometer generates individual cell size measurements, multiple samples can be overlaid on one histogram enabling analysis of the change in cell diameter over time.
Fig. 4 | Multiple Cell Images for Data Verification. With the Auto T4 Cell Counter, cell morphology can be immediately viewed on-screen. Two images with four fields of view each are captured per cell count. This is equivalent to the area of four quadrants of a hemocytometer. Users can confirm that: (1) Cells are counted correctly, based on size and shape, (2) Debris is excluded, (3) Cells within clumps are being counted individually, (4) Cell images can be archived and exported for use in publications and presentations, (5) Saved images can be re-counted using default or user-optimized analysis settings.
Fig. 5 | Exclusion of Debris. Because Cellometer recognizes cells based on size, brightness, and morphology, cellular debris is easily and accurately excluded from counting results.
Fig. 6 | 10x Faster and More Precise than Manual Counting. Counting 1 x 10⁶ cells takes approximately 5 minutes with a manual hemacytometer. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto T4 Cell Counter calculates cell count and concentration for live and dead cells and % viability in just 10 seconds. The Cellometer %CV (Coefficient of Variation), including sample preparation, was well under 5% for most cell lines tested. The CVs for manual counts ranged from 7% to 20%. Automated cell counters remove inter-operator variability and subjectivity from cell counts to generate more precise data.
|Cellometer Auto T4 Includes||
|Available Accessories||PC laptop|
|Number of Channels||1|
|Counting Speed Per Sample||<30 sec|
|Volume (per chamber)||20 µl|
|Cell Size/Diameter Range||5–60 µm (most mammalian cells)|
|Concentration Range||10⁵ – 10⁷ cells/ml|
|Software||Cellometer Auto Pattern-recognition software to quickly and accurately decluster, identify and count individual cells|
|Dimensions||W8.9 x D10.7 x H32.0 cm|
|Power Adapter||Input 100–240 VAC, 50/60 Hz, 0.5A; Output: 12 VDC, 1.5A|
|PC / Laptop Requirements
(If purchasing Cellometer without PC laptop)
|Product Name||Cat. No.|
|Cellometer Auto T4 Bright Field Cell Counter||Cellometer-Auto-T4|