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友善列印Cell Fractionation Kit - Standard

Abcam 所研發的 Cell Fractionation Kit - Standard (ab109719) 可以讓您以簡便快速的流程從細胞樣本中萃取出分別位在粒線體 (mitochondria)、細胞質 (cytoplasm) 與細胞核 (nuclear) 的蛋白質,特別適合需要觀察蛋白質在細胞內分布位置變化的科研人員使用。例如在下方圖 1 的 Western Blot (WB) 實驗結果中就可以觀察到 Jurkat 細胞、HeLa 細胞與 143B 細胞在加藥誘發產生細胞凋亡 (apoptosis) 後,細胞色素 c (cytochrome c) 的分布位置就從原本的粒線體 (M fraction) 轉移到細胞質 (C fraction)。

產品特點:

  • 可自細胞樣本中分離出位於粒線體、細胞質、細胞核的蛋白質。
  • 流程簡便快速,不需使用任何機械性外力,僅需簡單離心步驟就能完成。
  • 萃取產物可直接適用於 WB 或 ELISA 檢測。

Figure 1. Apoptosis was induced in Jurkat, HeLa and 143B osteosarcoma cells by treatment with staurosporine or in Jurkat cells by FAS. Mitochondrial and cytoplasmic fractions were isolated using Cell Fractionation Kit - Standard (ab109719) and probed using Cytochrome c Apoptosis WB Antibody Cocktail (ab110415). As is clear from the gels, cytochrome c has translocated partially in FAS-induced cells and STS-treated osteosarcoma cells, and almost completely in STS-treated Jurkat and HeLa cells. The three control targets allow for verification of the "cleanness" of the cell fractionation.

Figure 2. Cytoplasmic (C), mitchondrial (M) and nuclear (N) fractions of HepG2 cells were prepared as described in the protocol of Cell Fractionation Kit - Standard (ab109719). Fractions were analyzed by Western blotting using Cytochrome c Apoptosis WB Antibody Cocktail (ab110415) containing antibodies against mitochondrial matrix (pyruvate dehydrogenase subunit E1α, PDH E1α), mitochondrial inner membrane (F1-ATPase α (CV α)), mitochondrial intermembrane space (cytochrome c) and cytosolic (glyceraldehyde-3-phosphate dehydrogenase, GAPDH) markers as well as with antibodies against additional mitochondrial matrix (Hsp70) and nuclear (poly (ADP-ribose) polymerase, PARP and SP1) markers, followed by appropriate HRP-conjugated goat secondary antibodies and ECL detection.

若您有興趣進一步了解細胞各分部構造與胞器的指標性蛋白質專一性抗體產品,歡迎參考〈Subcellular and cell membrane markers - Antibodies against specific organelles, the cell membrane, or cytoskeletal components〉。

訂購資訊

Category Product Cat. No.
Cell Fractionation and Protein Extraction Kits Cell Fractionation Kit - Standard ab109719
Nuclear Extraction Kit ab113474
Mitochondria/Cytosol Fractionation Kit ab65320
Mitochondria Isolation Kit for Cultured Cells ab110170
Mitochondria Isolation Kit for Tissue (with Dounce Homogenizer) ab110169
Mitochondria Isolation Kit for Tissue (without Dounce Homogenizer) ab110168
Plasma Membrane Protein Extraction Kit ab65400
Lysis Buffers 10X RIPA Buffer ab156034
Native Lysis Buffer ab156035
10X Red Blood Cell (RBC) Lysis Buffer ab204733
Protease Inhibitor Cocktails Protease Inhibitor Cocktail ab271306
Protease Inhibitor Cocktail EDTA Free ab270055
Protease and Phosphatase Inhibitor Cocktail ab201119
Protease and Phosphatase Inhibitor Cocktail (EDTA-free) ab201120
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