||Example of CNMCS Protein Extraction from Mammalian Tissue. Total Protein and compartmental proteins extracted from rat colon tissue using Biochain’s Total Protein Extraction Kit and CNMCS Compartmental Protein Extraction Kit were submitted to SDS-PAGE in 5 identical gels. Coomassie staining of one piece of gel indicated distinct protein pattern of respective fractions. Immunoblotting of PVDF membranes transferred from 5 other pieces of gel against cytoplasmic marker protein GAPDH, nuclear marker protein Histone H1, membrane marker protein Na+/K+ ATPase, and cytoskeleton marker protein vimentin assigned the majorities of the marker proteins to their expected compartmental fractions.
Lane 1: Total Protein; Lane 2: Cytoplasmic Protein; Lane 3: Nuclear Protein; Lane 4: Membrane Protein; Lane 5: Cytoskeleton Protein.