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友善列印3-D Life Hydrogels

  • 操作容易迅速,約 90 秒內即可完成凝膠化反應 (gelation)。

  • 使用者可根據實驗需求,自行在 3D 培養基上接上感興趣的分子,譬如 RGD peptide 或是特定蛋白 (分子濃度最高可達到 5 mmol/L)。

  • 可自行調整膠體的堅硬度/柔軟度 (stiffness)。

  • 成分組成透明清楚 (defined composition),非動物來源。

  • 應用實驗包含:3D 細胞培養、Epithelial cyst, Spheroid 與 Colony 生成試驗、細胞遷移試驗 (migration)、細胞侵犯能力試驗 (invasion)。

 

操作流程簡單快速:

Schematic drawing of the three simple steps required to embed cells in a peptide-modified hydrogel.
  1. Polymer, buffer and peptide are mixed and incubated to covalently attach the peptide to the maleimide polymer.
  2. A cell suspension is added to the peptide-polymer conjugate solution.
  3. Cells and the peptide-conjugated polymer are mixed with crosslinker for gel formation and placed on a culture dish. After the gel is formed, culture medium is added and the culture dish is placed in an
    incubator for cell cultivation (not shown).

 

訂購資訊

Product Pack Size Cat. No.
Hydrogel Kit
3-D Life PVA-PEG Hydrogel Kit 1 Kit CEL-09-G-001
3-D Life PVA-CD Hydrogel Kit 1 Kit CEL-G81-1
3-D Life Dextran-PEG Hydrogel Kit 1 Kit CEL-G90-1
3-D Life Dextran-CD Hydrogel Kit 1 Kit CEL-G91-1
Hydrogel Components
3-D Life PEG-Link 200 µl CEL-L50-1
3 x 200 µl CEL-L50-3
3-D Life CD-Link 200 µl CEL-L60-1
3 x 200 µl CEL-L60-3
3-D Life Maleimide-PVA Set 1 Set CEL-M80-3
3-D Life Maleimide-Dextran Set 1 Set CEL-M90-3

相關產品 (點擊「產品名稱」,即可開啟詳細產品說明)

Product Pack Size Cat. No.
Adhesion Peptides
3-D Life RGD Peptide 1 µmol CEL-09-P-001
3 x 1 µmol CEL-P10-3
3-D Life Scrambled RGD Peptide 1 µmol CEL-09-P-003
3 x 1 µmol CEL-P11-3

 

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