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 3-D Life Hydrogels
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 操作容易迅速，約 90 秒內即可完成凝膠化反應 (gelation)。
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使用者可根據實驗需求，自行在 3D 培養基上接上感興趣的分子，譬如 RGD peptide 或是特定蛋白 (分子濃度最高可達到 5 mmol/L)。
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可自行調整膠體的堅硬度/柔軟度 (stiffness)。
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成分組成透明清楚 (defined composition)，非動物來源。
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應用實驗包含：3D 細胞培養、Epithelial cyst, Spheroid 與 Colony 生成試驗、細胞遷移試驗 (migration)、細胞侵襲能力試驗 (invasion)。
操作流程簡單快速：
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Schematic drawing of the three simple steps required to embed cells in a peptide-modified hydrogel.
- Polymer, buffer and peptide are mixed and incubated to covalently attach the peptide to the maleimide polymer.
- A cell suspension is added to the peptide-polymer conjugate solution.
- Cells and the peptide-conjugated polymer are mixed with crosslinker for gel formation and placed on a culture dish. After the gel is formed, culture medium is added and the culture dish is placed in an
incubator for cell cultivation (not shown).
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訂購資訊
| Product |
Pack Size |
Cat. No. |
| Hydrogel Kit |
| 3-D Life PVA-PEG Hydrogel Kit |
1 Kit |
CEL-09-G-001 |
| 3-D Life PVA-CD Hydrogel Kit |
1 Kit |
CEL-G81-1 |
| 3-D Life Dextran-PEG Hydrogel Kit |
1 Kit |
CEL-G90-1 |
| 3-D Life Dextran-CD Hydrogel Kit |
1 Kit |
CEL-G91-1 |
| Hydrogel Components |
| 3-D Life PEG-Link |
200 µl |
CEL-L50-1 |
| 3 x 200 µl |
CEL-L50-3 |
| 3-D Life CD-Link |
200 µl |
CEL-L60-1 |
| 3 x 200 µl |
CEL-L60-3 |
| 3-D Life Maleimide-PVA Set |
1 Set |
CEL-M80-3 |
| 3-D Life Maleimide-Dextran Set |
1 Set |
CEL-M90-3 |
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