ibidi µ-Slide Membrane ibiPore Flow — 流體環境細胞遷移試驗最佳研究利器

流體環境細胞遷移試驗最佳研究利器

針對流體環境細胞遷移試驗 (Transmigration under flow),伯森生技特別推薦由 ibidi 公司全新改版的µ-Slide Membrane ibiPore Flow,具備兩條可培養細胞的通道,通道交疊處採多孔玻璃膜設計,能將傳統使用 Transwell (Boyden chamber) 進行的各式 Transmigration & Transport 試驗轉移至流體環境下進行,模擬循環系統,更貼近生理環境!其產品特性包含:

  • 搭配幫浦 (例如 ibidi Pump) 不同剪切應力設定,可模擬生理上不同流體環境,如動脈、靜脈、肺泡等。
  • 玻璃膜具極佳光學性質,可輕易獲得清晰顯微鏡成像。
  • 針對不同細胞、不同應用,提供多種玻璃膜孔徑大小可選。
  • 符合標準載玻片規格 (75.5 x 25.5 mm),廣泛適用各類倒立式顯微鏡。

產品用途

  • 通過多孔膜的細胞遷移 (Transmigration)、趨化 (Chemotaxis)、侵入 (Invasion) 試驗。
  • 肺泡細胞或皮膚細胞微環境模擬。
  • 細胞極化試驗 (Apical-basal cell polarity assays)。
  • 細胞共同培養 (Co-culture)、血腦障壁 (Blood brain barrier) 模擬等等。

Transmigration under flow. Shown here is a time lapse movie of a transmigrating human HL-60 cell that was seeded into the lower channel of a µ-Slide Membrane ibiPore Flow (pore size 3 µm). Under flow conditions, the cell transmigrates in vitro through the porous glass membrane into the upper channel that was filled with a 3D collagen matrix containing the chemoattractant fMLP.

Transmigration under flow. Live cell imaging of a transmigrating single SiR-Actin-stained cell (indicated by pseudo colors) by spinning disc confocal microscopy (20x objective) with a time interval of 30 s. The arrow indicates the direction of flow at a shear stress of 1 dyne/cm²; Scale bar = 10 µm. Courtesy R. Pick, Munich, Germany.

Recommended pore sizes and porosities for different applications

依據細胞種類與應用實驗,選擇不同玻璃膜孔徑大小進行實驗

Product Name Pore Size Porosity Pack Size Cat. No.
µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat 0.5 µm 20% 10 pcs./box IB-85116
µ-Slide Membrane ibiPore 0.5µm/20% Flow ibiTreat, Sample Pack     2 pcs./box IB-85116-S
µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat 3 µm 5% 10 pcs./box IB-85126
µ-Slide Membrane ibiPore 3.0µm/5% Flow ibiTreat, Sample Pack     2 pcs./box IB-85126-S
µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat 5 µm 5% 10 pcs./box IB-85136
µ-Slide Membrane ibiPore 5.0µm/5% Flow ibiTreat, Sample Pack     2 pcs./box IB-85136-S
µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat 8 µm 5% 10 pcs./box IB-85146
µ-Slide Membrane ibiPore 8.0µm/5% Flow ibiTreat, Sample Pack     2 pcs./box IB-85146-S

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