3
其他
TSA訊號放大系統
Tyramide Signal Amplification
說明:
•功用:訊號放大,增加靈敏度,減少試劑使用量
•固相化的目標分子:DNA、RNA、Protein等欲分析的分子必須位在玻片(glass slides)、轉漬膜
(transfer membrane)或微量反應盤(Microplates)的表面。
•應用:Immunohistochemistry(IHC),In-Situ Hybridization(ISH),cDNA or Oligo Microarray,
Protein Array,ELISA,Western Blotting
•原理:利用目標分子團上的HRP酵素將帶有被標記過的Tyramide催化後形成自由基,此自由
基會與目標分子團附近的Tyrosine結合,如此每一個目標分子團上就有很多個被標記
過的Tyramide,而達到訊號放大的效果。請參閱下列的原理簡圖。
詳細原理圖請下載
TSA Principle
。
•相關文獻:請下載
TSA Reference List
產品種類介紹:
A. Western Blot及ELISA應用
目錄編號
產品
包裝
NEP116
ELAST ELISA Amplification system
10 x 96 wells
NEL761
BLAST (for Chromogenic Western Blotting)
2,500 cm2
NEL761A
BLAST (for Chromogenic Western Blotting)
500 cm2
B. 應用於IHC or ISH
目錄編號
產品
包裝 (No. of Slides)
NEL700
TSA Biotin
200-600
NEL700A
TSA Biotin
50-150
NEL701
TSA Fluorescein
100-300
NEL701A
TSA Fluorescein
50-150
NEL702
TSA Tetramethylrhodamine (TMR)
100-300
NEL703
TSA Coumarin
100-300
NEL704A
TSA Cyanine 3
50-150
NEL705A
TSA Cyanine 5
50-150
Kit components:
1. Streptavidin-HRP 2. Blocking reagent 3. Amplification Dilute 4. ***-Tyramide
C. TSA Plus DNP System應用於IHC or ISH
目錄編號
產品
包裝 (No. of Slides)
NEL746A
TSA Plus DNP (AP)
50-150
NEL746B
TSA Plus DNP (AP)
25-75
NEL747A
TSA Plus DNP (HRP)
50-150
NEL747B
TSA Plus DNP (HRP)
25-75
Kit components:
1. Anti-DNP conjugated with AP or HRP 2. Blocking reagent 3. Amplification Dilute 4. DNP-Tyramide
D. TSA Plus Fluorescence Systems應用於IHC or ISH
目錄編號
產品
包裝 (No. of Slides)
NEL741
TSA Plus Fluorescein
50-150
NEL741B
TSA Plus Fluorescein
250-750
NEL742
TSA Plus Tetramethylrhodamine (TMR)
50-150
NEL742B
TSA Plus Tetramethylrhodamine (TMR)
250-750
NEL744
TSA Plus Cyanine 3
50-150
NEL744B
TSA Plus Cyanine 3
250-750
NEL745
TSA Plus Cyanine 5
50-150
NEL745B
TSA Plus Cyanine 5
250-750
Kit components:
1. Amplification Dilute 2. ***-Tyramide
E. TSA Plus Multi-Fluorescence Systems應用於IHC or ISH
目錄編號
產品
包裝 (No. of Slides)
NEL752
TSA Plus Cyanine 3 / Cyanine 5
50-150 each dye
NEL753
TSA Plus Cyanine 3 / Fluorescein
50-150 each dye
NEL754
TSA Plus Cyanine 5 / Fluorescein
50-150 each dye
NEL756
TSA Plus Fluorescein / TMR
50-150 each dye
NEL760
TSA Plus Fluorescein, TMR, Cyanine 3 and Cyanine 5
10-35 each dye
Kit components:
1. Amplification Dilute 2. ***-Tyramide
F. TSA 應用在核酸晶片分析
目錄編號
產品
包裝
MPS521
Micromax TSA
10 reactions / 5 slides
MPS522
Micromax TSA
40 reactions / 20slides
MPS545
ASAP miRNA labeling Kit
10 reactions / 5 slides
進一步資訊請參閱
Microarray Probe Labeling System
G. TSA 應用在自動化的蛋白質晶片分析
目錄編號
產品
PAW8001
TSA Automated Cyanine 3 Base
PAW8005
TSA Automated Cyanine 3 Streptavidin
PAW8051
TSA Automated Cyanine 5 Base
PAW8055
TSA Automated Cyanine 5 Streptavidin
Kit components:
1. TSA Module Diluent Reagent 2. Cyanine 3 or Cyanine 5-Tyramide
3. 10x wash buffer 4. TSA module HRP Dilute
5. TSA Inactivation reagent 6. Blocking reagent
7. TSA Module bottle 8. Connector (4 total)
9. TSA Module Blocking Reagent