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核酸標定、純化與分析
序列分析樣本純化
(Sequencing Reaction Cleanup)
Montage SEQ Kit
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產品特色：
•能有效純化BigDyeTM Terminator v 3.0及DYEnamicO
ET Terminator的作用反應物
•有效增加定序結果的長度及訊號強度
•不需耗時的酒精沈澱與離心步驟，實驗流程縮短至
僅需10分鐘即可完成
• 96-well 及384-well format設計，適用於自動化系統
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簡易的操作流程：
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1.將sample放入well後，
加入injection solution |
2.以Manifold真空抽氣，
直至well完全抽乾為止 |
3. 以injection solution
wash sample一次後，
以injection solution
回溶取回純化後的
sequencing products
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良好的成效：
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Method |
Process Time |
Typical Phred 20 Values |
Average Pass rates |
| Montage
SEQ96 cleanup kit |
10 minutes |
680 |
>97 |
| Ethanol
precipitation |
80 minutes |
488 |
75 |
Competitor
Q 96-well gel filtration-
based dye terminator remove system* |
55 minutes |
630 |
94 |
| ﹡Protocol as per manufacture
recommendations. |
Sequencing was done omn plasmid template, 1/ 8th BigDye reactions,
and run on the ABI PrismTM 3700.
Results show that the Montage SEQ96 Sequencing Reaction Cleanup Kit delivers
the longest read lengths
and highest pass rates in less time.
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Montage SEQ384 kit purified
1/8th BigDye Terminator v 3.0 sequencing reactions analyzed
on the ABI Prism 3700.
This electropherogram further demonstrates the effectiveness
of Montage kits for dye terminator removed, delivering long,
high quality reads (Phred 20 = 715).
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